Basket Updated
0
Items added
A scientific resource for the BEACH protein domain containing information on structure, function, and phospholipid binding.
We recommend using a microwave to bring slides to a boil in 10 mM sodium citrate buffer, pH 6.0 and then to maintain at sub-boiling temperature for 10 minutes. This typically requires heating slides at full power for 2.5 -3 minutes, then adjusting to 20-30% power. After microwave heating, we cool the slides on the bench top for 30 minutes.
A scientific resource for the BTB/POZ protein domain containing information on structure, function, and domain binding during chromatin remodeling.
It remains difficult for scientists to predict the long-term health effects of COVID-19, particularly considering its diversity of effects during acute infection. The most severe impacts appear to manifest in tissues with high levels of vascularization (e.g., lungs, heart, kidney, liver), and it is evident that the virus elicits a significant inflammatory response in infected tissues
A scientific resource for the ANK protein domain containing information on structure, function, and binding partners.
Although we have performed extensive testing, we have not found an appropriate model to detect phospho-DRP1 (Ser637) in human samples. DRP1, when phosphorylated at serine 637, is very difficult to detect, as it is rapidly degraded under most conditions. The region of the DRP1 protein surrounding serine 637 is conserved between rat and human, therefore we would predict our phospho-DRP1 (Ser637) antibodies to detect the human protein. However, since we have not been able to obtain signal in human samples, we cannot validate or guarantee these antibodies for use with human samples.
Grant application guidelines and project description criteria for funding from CST.
A scientific resource for the ARM protein domain containing information on structure, function, and domain binding.
Cellular senescence is defined by permanent cell cycle arrest. Senescent cells accumulate with age and contribute to normal aging and age-related disorders
This sonication step is used simply to destroy the cellular and nuclear membranes, as the digested chromatin is still inside the permeabilized nucleus and needs to be released into the supernatant. This sonication step is done in 1xChIP Buffer which is not an optimal sonication buffer and will not further fragment the chromatin.
A scientific resource for the SH2 protein domain containing information on structure, function, and domain binding to phospho-tyrosine motifs.
Unfortunately, our PD-1 or PD-L1 antibodies have not been tested in neutralization assays. Therefore, we cannot guarantee their performance.
Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha
Short videos from CST highlighting our optimized western blotting protocol and troubleshooting guide to diagnose issues and recommend solutions.
CST's Western Blotting (WB) Troubleshooting Guide Video provides tips to help you diagnose experimental issues and offers recommendations to solve the problems.
Immunophenotyping is a technique that uses antibodies to allow the identification and quantification of particular cell types within a heterogeneous population.
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.1. A no lysate control. This includes the primary antibody at the highest concentration tested with samples containing only 0.1X sample buffer + Fluorescent Master Mix (FMM). This control will determine if there is any cross-reactivity between the primary antibody and the internal standards used in the assay.2. A no primary antibody control. This includes the antibody diluent (without antibody) plus the lysate used at the highest concentration. This control will help to rule out any cross-reactivity between the secondary antibody and the lysate.
We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with most cell lines. Depending on the cell type and the incubation time applied in the assay, 0.5 - 2x10^5 cells/well (or 100 ug/well of total lysate protein) is sufficient for most experimental setups. For best results, cell number or lysate concentration titrations are recommended. Because caspase-7 shares the same substrate sequence as caspase-3, this kit also detects caspase-7 activity.
Our Normal Goat Serum #5425 is not heat inactivated. If you are worried about enzymes influencing your experiment, heat inactivation is recommended. Incubating the Normal Goat Serum in a 56°C water bath for 30 minutes should be sufficient to inactivate the majority of restriction endonucleases.
CST's Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final results.
An interactive 3D molecular model depicting the cellular landscape of a nucleus and its various protein subsets including DNA Damage, Replication, Transcription, etc.
Our RAG1 (D36B3) Rabbit mAb #3968 was produced using a recombinant protein fragment with a sequence toward the N-terminal region of human RAG1. No significant homology is found in this region when aligning the RAG1 and RAG2 protein sequences. Therefore, this antibody should only cross-react with RAG1.
Listing of IHC-related scientific posters and papers involving scientists from Cell Signaling Technology.
Publications featuring Akt antibodies from Cell Signaling Technology.
The CUT&RUN Antibody Binding Buffer #15338, CUT&RUN 10X Wash Buffer #31415, and diluted CUT&RUN 1X wash buffer are stable at RT or 4C for one month. However, the Digitonin Solution #16359, Spermidine #27287, and Protease Inhibitor Cocktail #7012 can start to lose activity quickly if not stored at -20C. For the best performance, we recommend adding these components to the CUT&RUN Antibody Binding Buffer or CUT&RUN Wash Buffer on the day of the experiment and keeping them on ice during use.
We have several antibodies for detecting ribosomal protein S6 (rpS6) that are validated for IF-IC with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse mAb #2317. Both of these antibodies are suitable for immunofluorescence in mouse cells.
Protein-protein interactions form a network whose structure drives cellular function and whose organization informs all biological inquiry.