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Human samples will have 55 kDa and 25 kDa bands, representing the long and short isoforms (FLIP-L, FLIP-S). A third isoform, FLIP-R, was originally identified in Raji cells and migrates at approximately 25 kDa [see Djerbi, M. et al. (2001) Scand. J Immunol. 54, 180-9 (PMID: 11439165; https://www.ncbi.nlm.nih.gov/pubmed/1439165) and Golks A. et al. (2005) J. Biol. Chem. 280, 14507-13 (PMID: 15701649; https://www.ncbi.nlm.nih.gov/pubmed/15701649)]. Mouse FLIP is alternatively spliced, where only the long isoform is generated and, in some rare cases, the FLIP-R isoform may be observed. Frequently, the shorter isoforms undergo proteasomal degradation in mice and are not detected by western blot.
A scientific resource for the DED protein domain containing information on structure, function, and domain binding during apoptosis.
Expert-reviewed interactive pathway providing a current overview of Inhibition of Apoptosis Signaling.
Expert-reviewed interactive pathway providing a current overview of Death Receptor Signaling.
Cell behavior is a tightly regulated process where various signals work together to elicit a specific response. Unraveling these mechanisms allows us to understand how cells are regulated in a normal state.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
Sometimes understanding complex signaling is overwhelming. Try thinking about it as the wiring on a construction site. Maybe that will help!
Cell states can be monitored using markers correlating to your process of interest. These assays and antibodies can be used to detect cell viability, senescence, proliferation, apoptosis, cytotoxicity and oxidative state.
mIHC with tyramide signal amplification protocol. Detect and localize multiple proteins in tissue sections simultaneously. Click here to learn more.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
Necrosis has been classically defined as an unprogrammed form of cell death that occurs in response to overwhelming chemical or physical insult.
CST promotes alternative transportation through a variety of programs.
Activated by pro-inflammatory signaling, ischemic injury or viral infection, necroptosis is a programmed regulated form of necrosis requiring RIP3 and MLKL
Anti-CAR linker antibodies confirm CAR surface expression for CAR-T therapy, regardless of antigen specificity; 2023 winner of the CiteAb Innovation Award.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.
ELISA Protocol for PathScan Stress and Apoptosis Signaling Antibody Array Kit: easy to follow directions describing the step by step experimental procedure.
Compare Cell Signaling Technology's Cleaved Caspase antibodies against one another to determine optimal applications.
CST offers flow validated antibodies for single cell mass cytometry (MC) and IHC validated antibodies for imaging mass cytometry (IMC)
Expert-reviewed interactive pathway providing a current overview of T Cell Receptor (TCR) Signaling.
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Learn about the antibodies, custom reagents, and ready-to-use cell assay kits to support your CAR-T therapy development.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.
Stem cell markers and kits organized in table format: links to product pages; includes Oct-4, Sox2, Nanog, c-Myc, C/EBPα, LIN28 and KLF4.
