Basket Updated
0
Items added
We recommend using 10 mg of protein per immunoaffinity purification (IAP) sample for our standard PTMScan kits. For the PTMScan HS product line, 1 mg of starting material is optimal. The minimum sample amount is dependent upon the cell line/tissue and the endogenous levels of post-translational modifications (PTMs). If less protein is used, fewer identifications could be expected.
Immunofluorescence Protocol with Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.
Activated by pro-inflammatory signaling, ischemic injury or viral infection, necroptosis is a programmed regulated form of necrosis requiring RIP3 and MLKL
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
High-quality antibodies & assays supporting the interrogation of metabolic signaling pathways. Those required for energy, autophagy & biochemical reactions.
The California Supply Chains Act requires CST to disclose its current practices to combat slavery and human trafficking in five specific areas.
To perform intracellular flow cytometry, fixation and permeabilization of cells is required to allow for antibody penetration and subsequent binding to targets without disrupting cellular morphology.
Our ChIP assay kits avoid the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions. Learn more.
In our experience, digesting 4x10^6 cells yields 10-20 ug of chromatin, which is why this is mentioned in our ChIP FAQ. However, as stated in the protocols for our SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005, approximately 5ug - 10ug chromatin is required for ChIP depending on your target. In our experience, for histone (PTM) ChIP, as low as 1-2 ug of chromatin DNA is required per IP. For transcription factor ChIP, a minimum of 5-8 ug chromatin DNA is required per IP. For co-factor ChIP, a minimum of 10 ug chromatin is necessary per IP. This is why all of our validated antibodies have optimal dilutions based on 10ug per IP.
As long as the image is not being used for commercial use, we only require for you to acknowledge us with the text "Illustration reproduced courtesy of Cell Signaling Technology, Inc. (www.cellsignal.com)". This statement should be clearly represented with the image in all media.
Annual grant application guidelines and project description criteria for funding from CST.
The FastScan kits differ from the PathScan kits in that (1) they come with a positive control and (2) the assays can be performed much faster. With FastScan, the samples are incubated with a capture antibody conjugated with a proprietary tag and a detection antibody linked to HRP with no need for wash steps. The FastScan kits require the use of a specific lysis buffer, included in the kits, and are not compatible with other buffers. In terms of sensitivity, FastScan and PathScan are fairly equivalent.
No matter your research area, we’ve all needed to answer this deceptively easy question: how much of a particular protein is present in my samples?
Overview of GPCR, Calcium, cAMP Signaling Resources, interactive pathway diagrams, and other technical resources.
Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.
Study the PKC pathway's role in cellular signaling and regulation. Explore the molecular mechanisms & signaling events governed by this pathway. Click.
We recommend using a microwave to bring slides to a boil in 10 mM sodium citrate buffer, pH 6.0 and then to maintain at sub-boiling temperature for 10 minutes. This typically requires heating slides at full power for 2.5 -3 minutes, then adjusting to 20-30% power. After microwave heating, we cool the slides on the bench top for 30 minutes.
Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
A scientific resource for the PTB protein domain containing information on structure, function, and domain binding to phospho-tyrosine motifs.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Our Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) #9872 (both L and S sizes) is tested for sterility and endotoxin levels. All new lots are subjected to a 2 week sterility test and also tested to ensure an endotoxin level of less than 0.1 EU/ml PBS. We do not do any specific testing to certify the buffer as DNAse/RNase free, therefore we would not recommend using #9872 for experiments requiring RNAse free reagents.
Co-staining with multiple antibodies is a low-content form of multiplex analysis that be achieved using different host species or fluorophore conjugates.
The abstract, narrative, and facilities & resource and animal care statements are important parts of your grant that can determine whether you get funded.
CST antibodies undergo rigorous stability testing to ensure quality. To reduce our carbon footprint, cold shipping is used only when necessary.
Autophagy is more than just the bulk degradation of intracellular components. It can also selectively degrade specific organelles, pathogens, and proteins.
The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.