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Fluorescent Multiplex Immunohistochemistry (mIHC)

Fluorescent Multiplex Immunohistochemistry (mIHC)

Fluorescent Multiplex Immunohistochemistry (mIHC) allows for simultaneous detection of multiple proteins of interest on formalin-fixed paraffin-embedded (FFPE) tissue. One approach to fluorescent mIHC involves HRP-catalyzed deposition of tyramide-fluorophore conjugates in the vicinity or at the site of the antigen. This approach provides two key advantages: marked signal amplification and simplified panel design, wherein any IHC-P validated antibody regardless of host species or isotype can be used. CST offers thoughtfully pre-selected monoclonal antibody panels relevant to the study of tumor immunology. The panels are stringently validated for multiplex IHC and accompanied by a detailed and highly optimized protocol.

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