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A scientific resource for the PDZ protein domain containing information on structure, function, and domain binding.
A scientific resource for the POLO-Box protein domain containing information on structure, function, and domain binding to phospho-Ser/Thr motifs.
Cancer cells reprogram their metabolic pathways to enable energy production under conditions that are disabling to most normal cells.
Webinar | Post-translational Modification: Antibody Enrichment for Mass Spectrometry-based Proteomics
WEBINAR | Deciphering cancer: The intersection of epigenetics, metabolism, and tumorigenesis
A kinome diagram showing the ACG group of protein kinases from Cell Signaling Technology.
A scientific resource for the IQ protein domain containing information on structure, function, and domain binding to calmodulin.
Yes. In our protocol, we recommend a probe-based sonicator because it is less expensive and widely used in many labs. However, you can use a Covaris water bath sonicator. You will still need to optimize the sonication program based on your DNA image, since you want to avoid over-sonicating when performing sonication-ChIP as this can damage chromatin quality/integrity. When looking at sonication results, chromatin DNA fragments should appear as a smear typically ranging between 200 to 1000 bp in size. We recommend you use the condition that requires the minimal sonication cycles, as long as the majority of DNA is less than 1000bp.
Cellular senescence is a state of stable cell cycle arrest under which cells remain metabolically active, but no longer divide or respond to growth-promoting stimuli.
You can QC your CUT&RUN assay prior to NG-seq by performing qPCR analysis of your enriched DNA. Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis.
Guest blogger Dr Gregory discusses research to define the epitranscriptome, including modifications that increase tRNA stability and prevent degradation.
The antigenic peptides used to produce our IRAK4 Antibody #4363 and our IRAK-M Antibody #4369 have been carefully selected to avoid cross-reactivity with the other IRAK family members. Our IRAK-M Antibody #4369 can detect a faint band at approximately 55 kDa. However, although the IRAKs are closely related in sequence, this band is not IRAK4.
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy and light chain.
A scientific resource for the F-Box protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
While we recommend 1mg of tissue per assay in most cases, we have had some success with as little as 0.5 mg of tissue per assay. Easier tissue types, such as liver, or easier target types, such as histone modifications, require less tissue. Difficult tissue types like heart or difficult target types like some transcription factors or cofactors will likely require greater amounts of tissue, such as 2.5mg or 5mg.
A scientific resource for the CUE protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
A few photo highlights from the American Association for Cancer Research (AACR) Annual Meeting 2017 in Washington, D.C.
A scientific resource for the HEAT protein domain containing information on structure, function, and domain binding.
Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue
By using a pH that is a bit more basic and above the isoelectric point of a typical IgG, we can minimize antibody aggregation. This formulation is also conjugation-friendly.
IF-approved antibodies from CST are validated for use with a blocking buffer that contains 5% serum diluted in PBS containing 0.3% Triton X-100. Serum components offer diversity over alternatives such as milk and BSA and may bind and occupy more sources of non-specific protein-protein interaction. Choosing serum from the same species as the host species of your secondary antibody (e.g., goat, donkey) may also help to reduce background originating from endogenous Fc receptors. At CST, we offer fluorochrome-conjugated F(ab’)2 fragment secondary antibodies that reduce the potential for non-specific binding from this source. Therefore, if you are using one of our fluorochrome-conjugated F(ab’)2 fragment secondary antibodies, blocking may not be necessary.
A scientific resource for the GRIP protein domain containing information on structure, function, and domain binding to ARF family GTPases during vesicle trafficking.
A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.
Although we have performed extensive testing, we have not found an appropriate model to detect phospho-DRP1 (Ser637) in human samples. DRP1, when phosphorylated at serine 637, is very difficult to detect, as it is rapidly degraded under most conditions. The region of the DRP1 protein surrounding serine 637 is conserved between rat and human, therefore we would predict our phospho-DRP1 (Ser637) antibodies to detect the human protein. However, since we have not been able to obtain signal in human samples, we cannot validate or guarantee these antibodies for use with human samples.
This sonication step is used simply to destroy the cellular and nuclear membranes, as the digested chromatin is still inside the permeabilized nucleus and needs to be released into the supernatant. This sonication step is done in 1xChIP Buffer which is not an optimal sonication buffer and will not further fragment the chromatin.
Cellular senescence is defined by permanent cell cycle arrest. Senescent cells accumulate with age and contribute to normal aging and age-related disorders
Grant application guidelines and project description criteria for funding from CST.
A scientific resource for the ARM protein domain containing information on structure, function, and domain binding.
A scientific resource for the BEACH protein domain containing information on structure, function, and phospholipid binding.
We recommend using a microwave to bring slides to a boil in 10 mM sodium citrate buffer, pH 6.0 and then to maintain at sub-boiling temperature for 10 minutes. This typically requires heating slides at full power for 2.5 -3 minutes, then adjusting to 20-30% power. After microwave heating, we cool the slides on the bench top for 30 minutes.