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Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Tips to keep in mind when deciding between wet and semi dry transfer.
Studying neurodegenerative disease? Get the most from your sample: consider the research question being posed, the sample type, and available techniques.
Mitophagy is a well-studied example of selective autophagy. This post explores mitophagy functions and the consequence of excessive or inadequate mitophagy
A recent publication highlights the advantages and disadvantages of different approaches to multiplex IHC/IF and explains how these methods differ.
Episode 2 in the Responsible Science Podcast series. What are startups doing to contribute to responsible science?
All four of our MMP-2 antibodies should detect both the full-length proenzyme that runs at 72 kDa and the cleaved, active enzyme that runs at 64 kDa. As the activated form is secreted, it is more difficult to detect using endogenous models and often the 72 kDa proenzyme is more easily detected. In very high expression cell lines, such as U-87 MG, SF-296, and 3T3, the active form can be detected as a faint signal.
Discover the autophagy pathway and its crucial role in cellular homeostasis. Learn more and uncover the mechanisms of cellular self-degradation here.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
CST antibodies undergo rigorous stability testing to ensure quality. To reduce our carbon footprint, cold shipping is used only when necessary.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
Recombinant antibodies offer several key advantages: superior lot-to-lot consistency, continuous supply, and amenability to antibody engineering.
Enzyme Immuno Assay Kit Protocol: easy to follow directions describing the step by step experimental procedure.
Cell Signaling Technology (CST) complies with and is subject to the EU-U.S. Privacy Shield Framework and the Swiss-U.S. Privacy Shield Framework as set forth by the U.S. Department of Commerce regarding the collection, use, and retention of personal info
Specificity, consistency, and optimized assay conditions are three key elements that help ensure reliable immunofluorescence (IF) staining results each and every time.
We purchase our anti-IgM antibody from SouthernBiotech. It is Goat F(ab')2 Anti-Human IgM-UNLB (Cat. No.: 2022-01; https://www.southernbiotech.com/goat-f-ab-2-anti-human-igm-unlb-2022-01).
This post explores common T cell types, their roles in modulating immune cell response, and T cell activation via interaction with cell surface receptors.
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 because the RBD region ends at amino acid 527.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3.
PTMScan Motif Antibodies and Kits, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.
The preparation of a cell lysate is crucial to the success of many assays. Proper storage and handling of the lysate are crucial to avoiding degradation and maintaining the ability to detect your protein.
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
The antigen for the Rab11a Antibody #2413 surrounds Arg184 of the human Rab11a protein. The antigen only shares approximately 67% homology with the human Rab11b sequence, and is not expected to detect the Rab11b isoform.
Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.
What Assay should you be for Halloween? Western Blot, IHC, IF, Flow Cytometry, ChIP, or ELISA...
Cell Signaling Technology announces the launch of SignalStar™ Multiplex IHC, a flexible spatial biology solution for up to 8-plex amplification in two days.
A video tutorial illustrating the methodology behind PTMScan Technology and an example of its use in a recent phosphoproteomic study authored by scientists from CST.
Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).