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Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
New antibodies for Alzheimer's research were presented at SfN 2022, including tools to study neuroinflammation in glial cells like microglia and astrocytes
CST Technical Support Article
Protocol for PathScan Chemiluminescent Sandwich ELISA (for kits with Lyophilized Antibodies)
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
Specificity, consistency, and optimized assay conditions are three key elements that help ensure reliable immunofluorescence (IF) staining results each and every time.
PathScan Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.
SimpleChIP Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
Anti-CAR linker antibodies confirm CAR surface expression for CAR-T therapy, regardless of antigen specificity; 2023 winner of the CiteAb Innovation Award.
Custom-build antibody conjugates that match your assay requirements and deliver superior performance, and choose from a broad catalog of labels.
Tamar Aprahamian is an interesting study in the professional opportunities available for young scientists. She's worked in academia, industry, and most recently has co-founded a company of science writers called JetPub. She has been a contributor to our blog and was nice enough to share her story.
Because characterized antibodies are one reason that many experimental results cannot be replicated, researchers expect rigorous validation from manufacturers.
This product is purified from goat blood and is therefore 100% goat serum. If you are using it as a blocking buffer, you can dilute it as follows:Blocking Buffer: 1X PBS + 5% normal serum + 0.3% Triton™ X-100To prepare 10 mL, add 0.5 mL normal serum from the same species as the secondary antibody (e.g., Normal Goat Serum #5425 ) to 9.5 mL 1X PBS and mix well. While stirring, add 30 µL Triton™ X-100.
In this webinar, the speakers will explore how tumors exploit immune modulatory mechanisms to generate and thrive in their own immunosuppressive microenvironment, and explain how PD-1 immunotherapy stops certain cancers from turning off the anticancer immune response.
Interactive 3D molecular model depicting key adhesion signaling pathway nodes within their cellular landscape and integrated with PhosphoSitePlus protein pages.
Each GTPase Assay Kit provides enough reagents for 30 immunoprecipitations based on the amount of GST-fusion protein, Spin Cups, and Collection Tubes provided. The GTP and GDP is only enough for 10 assays. These two reagents are intended to be used as positive and negative controls alongside your own samples. For example, if working with a cell line treated with a compound to see if it would induce active Ras signaling (when using our Active Ras Detection Kit #8821, specifically), one would want to run these samples with GTP + GST-Raf1-RBD (positive control), GDP + GST-Raf1-RBD (negative control), and the samples + GST-Raf1-RBD to see if the treatment effectively induced active Ras compared to the positive control. In other words, GTP and GDP are usually not added to all 30 assays; 10 assays worth is typically an appropriate amount of these controls.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
We made our Leica Bond protocols available on the web, complete with validated primary antibody dilutions, so you can quickly load them into the Bond software.
PathScan Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.