Gene expression is modulated by post-transcriptional and translational control to regulate a variety of cellular processes, including development, cell differentiation, and metabolism. Post-transcriptional modifications and processing of messenger RNA (mRNA) regulates abundance and localization of mRNA molecules as well as downstream translation of mRNA to protein. The RNA life cycle is regulated by a multitude of RNA Binding proteins (RBPs) which are vital to ensure accurate and basic gene expression. Dysregulation of these RBPs and processes can result in a host of human diseases, such as cancer, neurodegenerative diseases including Alzheimer’s disease, spinal muscular atrophy, Amyotrophic Lateral Sclerosis (ALS), and autoimmune disorders. Partner with Cell Signaling Technology (CST) to obtain high-quality data through antibodies that examine RBPs and RNA modifications, in support of research toward understanding the processes governing the RNA life cycle.
RNA modifications are critical for post-transcriptional regulation of gene expression during multiple cellular processes, including development and cell differentiation. Recent advances have made it possible to investigate RNA modifications, however, obtaining high quality data requires accurate and validated antibodies. CST offers a variety of reliable antibodies for RNA modifications and associated writer, reader, and eraser proteins to support your research into the functions of RNA modifications.
Many types of RNA, including transfer RNA (tRNA), ribosomal RNA (rRNA), microRNA (miRNA) and long non-coding RNA (lncRNA) require modification or processing in order to perform their functions in the cell. For example, tRNAs are modified and conjugated to amino acids prior to functioning in protein translation. rRNAs are bound by RNA-binding proteins to assemble into ribosomes prior to protein translation. Multiple cellular functions and disease mechanisms are influenced by RNA processing. By offering a variety of validated antibodies for RNA binding proteins, CST is your expert source of reagents for investigations into understanding the mechanisms governing RNA processing.
In order for RNA to encode for a protein, it must be modified via capping, polyadenylation, and splicing before it can be transported into the cytoplasm for translation. Changes to this process can lead to genetic variation and increased disease risk. CST has a diverse catalog of products that can support your ability to monitor modulation of mRNA fate through alternative splicing, polyadenylation, and capping by RNA binding proteins.
Regulation of the stability and decay of RNA by surveillance mechanisms is vital to ensuring fidelity of genetic information flow. Disruption of this process results in a host of diseases ranging from neurodegeneration to cancer. CST provides a wide variety of reliable and validated antibodies for investigating the dynamics of RNA stability and decay as it applies to cell cycle processes, cellular differentiation, stress and immune responses.
Multiple classes of RNA can be transported from different locations, giving precise spatial and temporal control of gene expression in a cell. Localizing RNA is critical in many essential cellular functions including cell fate determination, directed cell movement, and tissue functionality. RNA visualization methods have revolutionized the investigation of RNA dynamics in cells. Let CST provide you the best validated antibodies to interrogate RNA transport pathways.
Translational control is a key component in essential cellular processes that maintain homeostasis - the disruption of which leads to human disease. The role of RNA in translational control supports the modulation of protein synthesis and protein factors which assist in metabolic function. CST provides a diverse and validated catalog of antibodies to probe mechanisms by which RNA and its proteins regulate translational control.
Our sampler kits have been carefully curated to obtain reliable data in your research evaluating multiple proteins involved in RNA regulation and translational control. These are an economical option for evaluating many aspects of gene expression by western blotting.
CST provides a wide-ranging catalog of ELISA assays and antibody duets to evaluate the essential role of RNA binding proteins in translation, transcription, and degradation, as well as gene expression. Our expert scientists can provide direction on designing an assay or choosing the right ELISA assay to interrogate RNA functions and the effects on physiology and pathology.