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CST Technical Support Article
CST scientists facilitate discovery and support investigator research by providing rigorously validated antibodies. Learn what it's like to work for us!
Find Pathways and Diagrams By Disease Area
CST offers custom-synthesized blocking peptides and AQUA peptides, including challenging sequences and unusual post-translational modifications.
Autophagy helps normal cells maintain homeostasis and acts as a survival mechanism in response to stress, where ULK1 plays an important role in signaling.
Validation for IF antibodies at CST Cell Signaling Technology
Technical support and customer service from Cell Signaling Technology.
Cell Signaling Technology Expands in China
Antibody formulations to match your assay and platform needs—custom BSA- and azide-free, formulate at higher concentrations, or modify into other buffers.
Non-neuronal cells like microglia and astrocytes play critical roles in maintaining proper neuronal function, development, and disease.
An outline and examples of the steps performed at Cell Signaling Technology to validate target specificity of an antibody for use in immunohistochemical analysis.
We use Microcon columns from Amicon (30 kDa cut off) to concentrate media. It is important that the medium does not contain FBS because this will make it difficult to concentrate with the Microcon columns.1. Spin the media (20 ml) to pellet any cellular debris.2. Add the 20ml volume to a Microcon column and concentrate it down to 500 ul.3. Add 10 ml of cold 1xPBS to the 500 ul sample to wash it and concentrate again to 500 ul.4. Transfer the 500 ul of concentrated media to an eppendorf tube and wash the Microcon tube membrane twice with 250 ul of cold 1xPBS to remove any proteins stuck to the membrane. This should result in a 1ml sample.5. Add 500 ul of 3x reducing SDS sample buffer to 1.0 ml of concentrated media. MMPs tend to be quite unstable and degrade easily in our hands, so we highly suggest adding protease and phosphatase inhibitors to the lysate, along with PMSF and 5 mM EDTA (as a chelator).6. Aliquot the lysates and store them at -80C.
I have a background in cancer biology and worked as a researcher at Massachusetts General Hospital and Novartis prior to joining CST in 2017. Today I’m on the other side of the bench, and while I’m not necessarily performing the research, I do feel like I’m directly contributing in my own CST way.
CST® Proteomics Analytical Services help you identify and validate drug targets, discover biomarkers, determine on/off-target effects, and mechanisms of action.
CST commitment to being your research partner: underscores this commitment with links to all available resources.
CST product packaging is based on feedback from customers as to the number of assays they typically run.
A listing of interactive 3D molecular models displaying signal transduction pathways in the context of their cellular landscapes produced by Cell Signaling Technology.
Cell Signaling Technology Awards $40,000 in Science Scholarships to Four North Shore High School Juniors
Technical support questions and issues for flow cytometry-validated antibodies and products from CST Cell Signaling Technology
3D spheroid models more accurately represent in vivo biological features and are being used in cancer research to mimic 3D tissue organization.
Technical support questions and issues for help with immunofluorescence-validated antibodies and products from CST Cell Signaling Technology
In-depth characterization of immune checkpoints in the tumor microenvironment
Application protocols: easy to follow directions describing the step by step experimental procedure.
Jan 2012, Ventana to collaborate with Pfizer & CST on companion diagnostic to identify lung cancer patients with ALK gene rearrangements.