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Expert-reviewed interactive pathway providing a current overview of Hippo Signaling.
Expert-reviewed interactive pathway providing a current overview of PTM Crosstalk Signaling.
Explore the mitochondrial apoptosis pathway and its role in programmed cell death. Read more about this essential pathway here.
Expert-reviewed interactive pathway providing a current overview of Cell Cycle G2/M DNA Damage Signaling.
Study the intricate cell cycle pathway's regulation of cell division and proliferation. Learn more here.
Expert-reviewed interactive pathway providing a current overview of SAPK/JNK Signaling.
A scientific resource for the 14-3-3 protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.1. It is very important to perform the methylene blue staining prior to blocking (Reference Step C in the DNA dot blot protocol).2. Stain the membrane with Methylene Blue Stain stain for 10 min at room temperature while gently shaking. Reagents we have had success with in our own internal testing include Methylene Blue Stain (MB 119, Molecular Research Center) and 1% Methylene Blue solution (M9140, Sigma, diluted in water).3. Wash the membrane, 2-3 times with dH20 (2-5 min each) until desired results are obtained.4. Once desired results are obtained, image blot immediately as the stain tends to fade.5. After imaging, proceed with blocking and antibody incubation with the membrane (step C). Remaining methylene blue should not affect antibody binding.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
Trademark information appearing on Cell Signaling Technology website.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
At CST, we use methanol-containing formaldehyde, specifically product F8775 from Sigma, to increase the shelf-life of our reagents. Therefore, it is not necessary to use methanol-free formaldehyde to cross-link your samples. Regardless of the type of formaldehyde used, it is necessary to ensure that the final concentration of formaldehyde is 1% in the plate or tube. It is worth noting that paraformaldehyde (PFA) is not a cross-linker and will result in a failed ChIP experiment.
For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation.
Validation using recombinant proteins or exogenous expression in a surrogate cell line can be used for targets with low levels of protein expression.
A categorical list of protein domain resources by Cell Signaling Technology. Each resource contains specific domain structure, function, and binding information.
KinomeView® Profiling Kit and Services
PhosphoScan® Phosphorylation Proteomics
Expert-reviewed interactive pathway providing a current overview of Cell Cycle G1/S Checkpoint Signaling.
The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench and share tips, tricks, and information.
Expert-reviewed interactive pathway providing a current overview of Contribution of Extracellular Matrix to EMT Signaling.
Study the intricate machinery of the apoptosis signaling pathway. Click here to learn more and gain insights into programmed cell death mechanisms.
Interact with this diagram showing the epigenetic regulators of Histone H3 including those responsible for monomethylation, asymmetric and symmetric dimethylation, and trimethylation.
Testing ELISA kit lots for percent coefficient of variation (CV), signal/blank ratios, and optical density helps ensure scientific reproducibility.
Expert-reviewed signaling pathway providing a current overview of adherens junction dynamics and links to products by Cell Signaling Technology.
Immunohistochemistry (IHC) is a powerful technique. Learn how to perform IHC on paraffin sections with our step-by-step IHC protocol. Click here.
Interact with this diagram showing the epigenetic writers and erasers of histone H2A, histone H2B, and Histone H4, including the involvement of acetyltransferases, deacetylases, methyltransferases, demethylases and kinases.
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
While healthcare providers and researchers race treat COVID-19, the quality of the tests themselves threatens to undermine their progress.
