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Expert-reviewed interactive pathway providing a current overview of ATP-Dependent Chromatin Remodeling Complexes.
Expert-reviewed interactive pathway providing a current overview of Protein Acetylation.
A scientific resource for the LIM protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.
A scientific resource for the Chromo protein domain containing information on structure, function, and binding to methylated lysine residues during chromatin remodeling.
Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.
The Caspase-4 Antibody #4450 recognizes an epitope that is present in both the full-length protein and the p20 subunit. Therefore, this antibody is predicted to detect both targets. However, we have not confirmed the reactivity of the Caspase-4 Antibody #4450 with the p20 subunit at endogenous levels. The exact protein load of recombinant, active caspase-4 shown in the western image on the product datasheet for the Caspase-4 Antibody #4450 has not been recorded. However, we typically load 1-5 ng of recombinant protein for such experiments.
A scientific resource for the BH protein domains containing information on structure, function, and domain binding in apoptosis.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
Immunofluorescence Protocol with Image-iT FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.
A scientific resource for the SPRY protein domain containing information on structure, function, and domain binding.
Interact with this signaling pathway showing immune checkpoint regulation in the tumor microenvironment, including targets such as PD-L1, GITR, TIM-3, CTLA-4, OX40, and more.
PathScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.
Learn how to use our formaldehyde fixation protocol on your samples to preserve morphology and antigenicity. An easy to follow step-by-step procedure.
Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.
Protocol for PathScan Chemiluminescent Sandwich ELISA (for kits with Lyophilized Antibodies)
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
Learn more about stem cell pathways and the transcription factors Oct-4, Sox2, and Nanog are essential for maintaining the pluripotent state of stem cells.
FastScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.
Immunofluorescence Protocol with Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
iPRM is a multiplexed LC/MS assay service that combines the specificity of mass spectrometry, the throughput of an immunoassay, and the power of multiplexing to accelerate the time-to-market of your drug and/or diagnostic test.
PathScan Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.