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It is possible to see a doublet at ~100 kDa for Grp94 due to post-translational modifications. Here is a link to our PhosphoSitePlus database detailing the possible post-translational modifications for Grp94: https://www.phosphosite.org/proteinAction.action?id=6928&showAllSites=true.
Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.