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A recent publication highlights the advantages and disadvantages of different approaches to multiplex IHC/IF and explains how these methods differ.
Products and Related Resources for Cytokines and Inflammation SARS-CoV-2 Research
Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
There are pros and cons to using monoclonal antibodies instead of polyclonals, and the same is true for using antibodies from rabbit hosts over mice hosts.
PathScan Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.
Protocol for PathScan Chemiluminescent Sandwich ELISA (for kits with Lyophilized Antibodies)
Western Blotting Protocol (Fluorescent): easy to follow directions describing the step by step experimental procedure.
Step-by-step ChIP assay protocol (agarose beads). A powerful technique used to study protein-DNA interactions, identify regulatory elements. Learn more.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Western blot procedure, from separating proteins on a gel to transferring them to a membrane and detecting them with antibodies. Click here.
Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
PathScan Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
Caspase-3 Activity Protocol: easy to follow directions describing the step by step experimental procedure.
FastScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
Fostering mentorship, application-based learning, and a love of science are all part of the mission of the CST internship program. Students interning with us are partnered with CST Mentors.
A troubleshooting guide for CUT&Tag–a ChIP-seq alternative with lower sample requirements, lower sequencing cost, and improved signal-to-noise for profiling chromatin and protein-DNA interactions.
Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
PathScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
BrdU Cell Proliferation Chemiluminescent Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
