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Our Cleaved PARP (Asp214) (D64E10) XP Rabbit mAb #5625 is designed to be specific for the large fragment (89 kDa) of human PARP1 protein that is produced by caspase cleavage at Asp214. As such, it specifically recognizes an epitope at the N-terminus of the large fragment that only becomes exposed upon caspase cleavage. Therefore, #5625 will not recognize the c-terminal PARP-1 fragments (55 and 42 kDa) that are generated during necrosis by lysosomal proteases [see Gobeil, S. et al. (2001) Cell Death Differ. 8, 588-94 (PMID: 11536009; https://www.ncbi.nlm.nih.gov/pubmed/11536009)].Unfortunately, we do not offer a PARP-1 antibody that will recognize both of the c-terminal PARP-1 fragments (55 and 42 kDa). Our PARP Antibody #9542 will not recognize either fragment; whereas, our PARP (46D11) Rabbit mAb #9532 may recognize the 55 kDa fragment, but not the 42 kDa fragment.
Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
Discover the DNA damage repair pathway & its critical role in maintaining genome integrity. Gain insights into the mechanisms driving these processes.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
Learn about the differences between BRCA1 and BRACA2 gene mutations and the search for new therapies targeting DNA repair pathways beyond PARP inhibitors.
We have several antibodies for cleaved PARP that are validated for IHC-P in human samples. These are Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 and Cleaved-PARP (Asp214) (E2T4K) Mouse mAb #32563. Both work well and there is no preference for this application.
Mutations in cell death pathways, such as apoptosis, mitophagy, necroptosis, and autophagy, contribute to neuronal cell death and the progression of neurodegenerative diseases.
Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research
Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.
Find out the best control cell extracts to use as positive or negative controls in your western blots, when studying apoptosis, autophagy, and mitophagy.
Overview of apoptosis signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources for apoptosis research.
Apoptosis is a highly regulated form of programmed cell death that occurs in multicellular organisms throughout life and in response to cellular stress.
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research