Resources results for "TAK1"
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We create the motif logos for our PTMScan product datasheets by running a bioinformatics workflow that takes the primary peptide sequence from a PTMScan IAP and extends this -7 and +7 amino acids from the PTM site. This generates a unique list of sequences with the PTM of interest in the 0 position. We then input this list into the SiteLogo tool in PhosphoSitePlus (https://www.phosphosite.org/sequenceLogoAction) to generate the motif logo.
Resources
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&RUN assay. Alternatively, Peter J. Skene et al. (2017; PMID28079019) suggest that you may want to try an antibody validated for an immunofluorescence assay, as the antibody binding in CUT&RUN takes place in an intact nuclear environment, resembling conditions for antibody binding in immunofluorescence assays.