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A scientific resource for the LIM protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.
We're looking ahead to a big 2020. Here's a look at what you'll see on Lab Expectations.
The adaptive immune response uses antigen-specific receptors to detect foreign antigens with help from T cells, B cells, and NK T cells.
The concentration of CaCl2 in the CUT&RUN Assay Kit #86652 is 100mM.
The samples shown in the web image for our Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833 were prepared as follows:1. Treat cells with 200 nM Doxorubicin for 24 hrs, then aspirate media and replace with fresh growth media. Rinsing with a small amount of the growth media to remove all old media prior to adding the final volume for the rest phase is recommended, but not necessary.2. Allow the cells to rest for 3 days. The 3 day rest period is necessary as it takes time to build up β-Galactosidase levels in the cells.
Humans have been creatively reusing materials for centuries. As our global waste problem grows, we must be more creative and diligent in finding solutions.
Meet the aspiring young scientists who won the 4th annual Rising Black Scientists Awards essay competition.
It is important to use the RCF (relative centrifugal force) settings on your centrifuge rather than the RPM (revolutions per minute) settings when using the Cell Fractionation Kit #9038 as RCF accounts for the size of the rotor and is therefore a more accurate representation of the force being applied to the sample.
The reagents provided with our Senescence β-Galactosidase Staining Kit #9860 are sufficient to stain 125 x 35 mm wells. The volumes listed in our protocol are intended for one 35 mm well in a 6-well plate with the guideline that the volumes should be approximately half the recommended volume of tissue culture media (e.g., 1 mL of β-Galactosidase Staining Solution when 2 mL of media is recommended). The volumes in the protocol can be adjusted based on this guideline and on the size/growth area (cm2) of the plate you plan on using for your experiment.
Dec 2011, CST wins "Best Performing Antibodies" and "Best Breakthrough Products Cancer Research" Categories in Life Science Industry Awards.
TET2 is post-translationally modified (https://www.phosphosite.org/proteinAction.action?id=22594&showAllSites=true). Although the theoretical molecular weight is 223.8 kDa these modifications increase the molecular weight so that it is usually observed at ~ 280 kDa. Comparison testing has been performed with our TET2 (D6C7K) Rabbit mAb (Mouse Specific) #36449 and TET2 Antibody #45010 (approved for human, mouse, and monkey) and the bands migrate at the same molecular weight for both antibodies. The specificity of #45010 was tested with HEK parental and TET2 knock-out cell lines (see datasheet) and the observed target band was confirmed to be TET2. This validates the observed band size that we see with our TET2 antibodies, including #36449.
Human samples will have 55 kDa and 25 kDa bands, representing the long and short isoforms (FLIP-L, FLIP-S). A third isoform, FLIP-R, was originally identified in Raji cells and migrates at approximately 25 kDa [see Djerbi, M. et al. (2001) Scand. J Immunol. 54, 180-9 (PMID: 11439165; https://www.ncbi.nlm.nih.gov/pubmed/1439165) and Golks A. et al. (2005) J. Biol. Chem. 280, 14507-13 (PMID: 15701649; https://www.ncbi.nlm.nih.gov/pubmed/15701649)]. Mouse FLIP is alternatively spliced, where only the long isoform is generated and, in some rare cases, the FLIP-R isoform may be observed. Frequently, the shorter isoforms undergo proteasomal degradation in mice and are not detected by western blot.
Depending on the target, the FastScan™ ELISA Cell Extraction Enhancer Solution #25243 usually increases the sensitivity/signal and performance of the kit. Therefore, if it is omitted, your signal may be lower, however the extent of this effect will vary from kit to kit. We recommend using the enhancer to avoid this possibility. If you forgot to add the enhancer to the FastScan™ ELISA Cell Extraction Buffer #69905 that was used to make the sample lysate, you can spike in the 50x Enhancer solution afterwards (and always add the enhancer to the extraction buffer that is used to dilute the samples). We believe the enhancer increases the signal by helping increase the recognition and binding of the target protein by the antibodies in the kit.
Knowing how antibodies and immunoassays have evolved as research tools can help you design more robust, reproducible scientific experiments.
Much has been written about the hallmarks of cancer, but we expand on the story with The Guide to the Hallmarks of Cancer Research Targets.
Learn about antibody diversity, host species, isotypes, and how antibodies are developed for research, clinical diagnostics, and therapeutic uses.
Cell Signaling Technology (CST) talks March for Science and what we can do to stay green this upcoming Earth Day.
This post summarizes the findings in the Nature paper YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer.
Lymphoid lineage cells can be characterized using antibodies for cell type-specific markers, allowing cells to be visualized using flow cytometry or IHC.
Discover the key RNA binding proteins (RBPs) in the lifecycle of messenger RNA (mRNA)
World Cancer Day raises awareness for cancer prevention, detection, and treatment. Learn about the scientific breakthroughs that are closing the care gap.
Learn about host-based and dye-conjugated methods for IF multiplexing, as well as a sequential labeling strategy that works with indirect detection.
In an effort to curb carbon emissions, highway congestion and promote employee wellbeing, CST drafted an Alternative Transportation Plan (ATP).
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
The regulation of ER stress and autophagy during viral infection is an important factor in the balance of virus and host survival.
Loading control proteins are often more abundant than the protein of interest, which can lead to the signal exceeding the linear dynamic range of detection
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
These antibodies to CNS markers can help study how LRRK2 mutations impact the autophagic-lysosomal pathway (ALP) and contribute to Parkinson's disease.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
