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Changing the primary antibody dilution and incubation conditions affects fluorescence intensity and signal-to-noise ratio in IF experiments.
There may be some lot-to-lot variability in the signal strength of our Vimentin (V9) Mouse mAb #49636 when testing by western blot due to this antibody being sold by weight instead of reactivity.
An outline and examples of the steps performed at Cell Signaling Technology to validate target specificity of an antibody for use in immunohistochemical analysis.
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
Learn how to visualize protein expression in tissue with immunohistochemistry (IHC). See examples of successfully optimized IHC assays.
Researchers used to have to confirm pluripotency using functional assays, but can now use IHC markers to assess pluripotency and differentiation capacity.
Discover the PI3K Akt pathway and its crucial role in cell growth and survival. Click here to learn more & gain insights into this important signaling cascade
Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.
For years, the TME could only be viewed in single-cell snapshots by flow cytometry or limited tissue staining. IMC offers a panorama of this dynamic world.
Can a cellular identity crisis lead to cancer progression?
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Read about the project to validate CST antibodies for spatial biology research on the Leica Cell DIVE, which can image 60+ biomarkers in one tissue sample.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.