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Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research
Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
Case Study 3: Exceptional Reproducibility of XP Monoclonal Antibodies from Cell Signaling Technology.
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Autophagy is more than just the bulk degradation of intracellular components. It can also selectively degrade specific organelles, pathogens, and proteins.
We recommend a 1 hour incubation at room temperature. We have found that prolonged incubation (>2hrs) and elevated incubation temperatures (>25C) can promote non-specific binding.
Regrettably, we do not test our Streptavidin-HRP #3999 for use in ELISA . Therefore, we cannot provide any firm guarantees or a recommended dilution. However, it should work for ELISA with optimization. We suggest starting with a 1:2000 dilution (0.1 µg/mL).
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
CST is a proud member of the 1% for the Planet organization, giving annually to benefit social and environmental causes.
If your DNA fragments are larger than 500 base pairs, we recommend sonicating to form 200-500 base pair fragments that will more readily denature. Sonication should be performed prior to starting the MeDIP or dot blot protocol. DNA fragment size can be analyzed by gel electrophoresis on a 1% agarose gel with a 100 bp DNA marker.
Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
CST believes in a data driven approach to continuous improvement and implementation of business practices that minimize negative impact on nature.}{icon: sustainability
Learn about the differences between BRCA1 and BRACA2 gene mutations and the search for new therapies targeting DNA repair pathways beyond PARP inhibitors.
Many researchers believe higher antibody concentration leads to better results—but that’s not true. Using antibodies at a specified dilution can give better results.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench and share tips, tricks, and information.
Protein-DNA interactions mediate epigenetic processes. Researchers studying these interactions need a means of analyzing when & where they occur on the genome.
SimpleChIP Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
Step-by-step ChIP assay protocol (agarose beads). A powerful technique used to study protein-DNA interactions, identify regulatory elements. Learn more.
In the CUT&RUN kit protocol, the addition of digitonin to the buffers facilitates the permeabilization of cell membranes & the entry of the primary antibody & pAG-MNase enzyme into the cells & nuclei.
Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.