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Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research
A scientific resource for the TUBBY protein domain containing information on structure, function, and domain binding to DNA and phospholipids.
A scientific resource for the CALM protein domain, also known as ANTH domain, containing information on structure, function, and binding to phospholipids.
A scientific resource for the ENTH protein domain containing information on structure, function, and domain binding to phospholipids during endocytosis.
A scientific resource for the PX protein domain containing information on structure, function, and domain binding to phospholipids.
Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.
A scientific resource for the PH domain containing information on structure, function, and domain binding to phospholipids during protein trafficking and cell signaling.
A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.
Discover the phosphoinositide pathway's role in cellular signaling and membrane dynamics. Click here to read more about PI signaling here.
A scientific resource for the GLUE protein domain containing information on structure, function, and binding to phospholipids in the regulation of vesicle trafficking.
A scientific resource for the FERM protein domain containing information on structure, function, and domain binding in cytoskeleton-associated proteins.
A scientific resource for the GRAM protein domain containing information on structure, function, and phospholipid binding during vesicle trafficking.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development
Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications
We understand some customers choose to pursue TMT labeling for PTMScan proteomics analysis, but we do not have a defined protocol for this method. However, there are two questions we can address:
1) We know that labs have succeeded in performing this experiment by enriching for the PTM peptides first then labeling second. This is critical for UbiScan and ubiquitin remant in particular because the TMT label is now on the K-e-GG remnant and the antibody won't be able to bind if the labeling is performed first.
2) We have recently found that to recover the TMT peptides efficiently from the stage tip C-18 desalting step you need to use 50% ACN/ 0.15% TFA (an increase from 40%). The TMT label makes the peptides more hydrophobic and the increase in ACN helps elute them from the C-18.
Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.
Cellular senescence is stable cell cycle arrest linked to aging and cancer and other disease states, including those associated with inflammation. It is induced by DNA damage, oncogenic signaling, and telomere shortening.
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
Interactive p38 MAPK pathway. Learn how p38 MAPK is involved in regulation of HSP27, MAPKAPK-2, MAPKAPK-3, & several transcription factors.
Epigenetic regulation, including aberrant DNA methylation and histone modifications, have been linked to Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, and amyotrophic lateral sclerosis.
Interested in studying senescence? Understanding cell cycle arrest is critical to many fields of research, including development, aging, and cancer.
Cellular senescence is characterized by irreversible cell-cycle arrest in combination with a distinct secretory phenotype and expanded lysosomes in response to stress. Senescent cells accumulate in tissues during aging, and various markers of senescence
Resources for the study of Translational Control, Protein Synthesis, and RNA Regulation including antibodies and associated reagents and interactive pathway diagrams.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
Numerous p53 splice variants have been documented in the literature. They arise from alternative splicing of p53 transcripts and result in multiple isoforms. UniProt for the human sequence (UniProt P04637) lists 9 isoforms produced by alternative promoter usage and alternative splicing and there may be more, depending on the model. Some of these splice variants are associated with polymorphisms or mutations of the p53 genes. Isoform 1 (43.6 kDa) is predominant. However isoform 2 (37.8 kDa) and isoform 3 (38.5 kDa) are also well documented. The combination of p53 cleavage and alternative splicing allows for a diverse size-range of p53 proteins, however, the study of p53 degradation has focused largely on the canonical full-length isoform 1. Also note that the proline-rich region of p53 slows the migration of p53 in SDS-PAGE gels, increasing the apparent molecular weight of the p53 isoforms that express it. [see M.P. Khoury and J.C. Bourdon. (2010) Cold Spring Harb Perspect Biol. 2(3):a000927, Review (PMID: …
Exactly what is ELISA? Learn about the enzyme-linked immunosorbent assay, the specific interaction between antibody & antigen. Click here.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).