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Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development
A scientific resource for the HEAT protein domain containing information on structure, function, and domain binding.
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
This guide includes some of the most common markers used to detect neuronal and glial cells, which include oligodendrocytes, astrocytes, and microglia.
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
CST Scientist Richard Cho shares his perspective on the 2017 Sf meeting, including new research findings about non-neuronal cells in the brain.
Expert-reviewed interactive pathway providing a current overview of the molecular and cellular biology of Alzheimer’s Disease.
Immunohistochemical Analysis. Learn the best ways to interpret IHC staining and understand experimental results accurately. Click here.
How do you design a panel of antibodies and fluorophores for mIHC? Learn how to set up a protocol that avoids epitope masking by optimizing antibody order.
Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Image-iT FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.
Identify microglia by using these antibodies, which are designed to detect protein biomarkers specifically expressed within these cells.
ProLong® Gold Antifade Reagent with DAPI #8961 will not freeze solid at -20C. It does, however, become very viscous, and we recommend bringing it to room temperature before pipetting. Freeze-thaw cycles do not appear to affect the efficacy of this product. Additionally, the product is stable for up to 6 months at room temperature, so therefore it is safe to assume it is stable for the same period of time at 4C.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
SignalStar™ is a tool for studying FFPE tissue to identify what cell types are present, their spatial location, and their functions. Learn more here.
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spectra of DRAQ5 and DyLight 680 overlap, and the signal produced by each dye cannot be distinguished.
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.
Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment.
Immunotherapy harnesses the power of the immune system to fight immunodeficiences, autoimmune diseases, and cancer. Manipulating the immune system to attack harmful cells is an area of intense research.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.
SignalStar™ Spatial Profiling is a revolutionary new tool for spatial biology research with fully customizable and highly-validated antibody IHC panels.
These markers can be used to study necroptosis and pyroptosis, forms of regulated necrotic cell death that share morphological features with necrosis.
