Resources results for "NR2F2"

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CD Markers

A list of important CD markers that includes links to PhosphoSitePlus, Uniprot, and products from Cell Signaling Technology.

Chasing Tau-biomarkers for Alzheimer’s Disease [Part 1]

There are no tools or therapies to cure, prevent, or slow the progression of AD. Current therapeutic approaches include targeting amyloid plaques and neurofibrillary tangles.

Publications By Our Scientists

A comprehensive list of peer-reviewed publications from Cell Signaling Technology.

Ubiquitin Ligase Table

The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).

Rising Black Scientists Awards 2023: Presenting the 3rd Annual Essay Competition Winners

Meet the winners of the 3rd annual Rising Black Scientists Awards, an essay competition that supports talented Black American scientists on their journeys.

The larger NRF2 size is likely due to a secondary structure or modification, which is not uncommon. Under normal conditions, KEAP1 targets NRF2 for degradation through the polyubiquitination/proteasome pathway, which makes it hard to detect the protein in cells (some cell lines have mutations in the NRF2 pathway that may cause NRF2 accumulation). After certain treatments, KEAP1 is modified and cannot bind NRF2, so NRF2 is not polyubiquitinated and will accumulate and enter the nucleus to activate transcription of its targets. Because polyubiquitination may result in different numbers of ubiquitin molecules binding to the same target protein, most polyubiquitinated proteins will not appear as distinct bands on western blot, but as a smear or ladder in some cases.

Resources

NRF2 can be a tricky protein to work with. Under basal conditions, the protein is marked for ubiquitin-mediated degradation in the cytoplasm and is therefore difficult to capture in the cell lysate. In order to detect this target in cell lysates, we typically treat the cells with a compound or chemical modulator to prevent the degradation of NRF2. For QC purposes, we treat cells with MG-132, #2194, a proteasome inhibitor, which helps prevent degradation and allows for accumulation of NRF2 within the cell.

Mechanisms of Cell Death: Ferroptosis

Ferroptosis is a form of programmed cell death characterized by condensed mitochondria, reduction in mitochondrial cristae, and increased membrane density.

ChIP–qPCR Validated Antibodies

Protein-DNA interactions mediate epigenetic processes. Researchers studying these interactions need a means of analyzing when & where they occur on the genome.

Overview of Metabolism and Metabolic Disorders

Metabolism defines all of the chemical changes or processes within cells, tissues, and organisms that sustain cellular homeostasis.

Nuclear Receptors

The nuclear receptor signaling plays important roles in cell differentiation, proliferation, metabolism, and other physiological processes. Click here.

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

Matched Antibody Pairs

Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.