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A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.
Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.
Expert-reviewed interactive pathway providing a current overview of Regulation of Actin Dynamics.
Do ChIP-validated antibodies work in CUT&RUN assays? Not necessarily. We found only 50-60% of ChIP- or ChIP-seq antibodies were compatible with CUT&RUN.
Immunohistochemistry Leica Bond Protocol: easy to follow directions describing the step by step experimental procedure.
The composition of our Lysis/Binding/Wash Buffer #11524 is as follows: 25 mM Tris-HCl, pH 7.2, 150 mM NaCl, 5 mM MgCl2, 1% NP-40 and 5% glycerol.
The CUT&RUN Antibody Binding Buffer #15338, CUT&RUN 10X Wash Buffer #31415, and diluted CUT&RUN 1X wash buffer are stable at RT or 4C for one month. However, the Digitonin Solution #16359, Spermidine #27287, and Protease Inhibitor Cocktail #7012 can start to lose activity quickly if not stored at -20C. For the best performance, we recommend adding these components to the CUT&RUN Antibody Binding Buffer or CUT&RUN Wash Buffer on the day of the experiment and keeping them on ice during use.
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry (IHC) is a powerful technique. Learn how to perform IHC on paraffin sections with our step-by-step IHC protocol. Click here.
ELISA Protocol for PathScan Stress and Apoptosis Signaling Antibody Array Kit: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.
Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.WaLP recognizes and cleaves SUMO proteins containing AGG, SGG, TGG or VGG motifs at the SUMO c-terminus.One should verify that the predicted SUMO sequence from their model organism contains these motifs at the c-terminus.
IHC protocol for frozen sections. This protocol covers everything from sample preparation to labeling & visualization. Click here.
Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
BrdU Cell Proliferation Chemiluminescent Protocol: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060: easy to follow directions describing the step by step experimental procedure.
PathScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
BrdU Cell Proliferation Protocol: easy to follow directions describing the step by step experimental procedure.