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CST antibodies have been validated for Simple Western, the only platform on the market that provides a fully automated western blotting workflow.
Overview of Angiogenesis antibodies & related reagents, interactive pathway diagrams, and other technical resources.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
Learn here about the HIF-1 signaling pathway and its impact on oxygen homeostasis and cellular adaptation. Click here.
This interactive guide shows the most important cell and functional state markers for tumor-infiltrating immune cells in mice.
A scientific resource for the PDZ protein domain containing information on structure, function, and domain binding.
Expert-reviewed interactive pathway providing a current overview of angiogenesis pathways and links to products by Cell Signaling Technology.
Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.
Explore the AMPK pathway and its role in cellular energy homeostasis. Uncover the mechanisms of energy regulation. Learn more here.
Jan 2013, CST granted patent for PCR Methods for detection of a subset of treatable non-small cell lung cancers.
Cell Signaling Technology, Inc. Announces License in Personalized Cancer Diagnostics.
To study pathways regulated by nitrotyrosine, CST offers two recombinant monoclonal antibodies against the same PTM for WB and proteomic applications.
Metabolic processes regulate immune cell function in a process known as immunometabolism, which can be studied using these macrophage and T-cell markers.
The PI3K / Akt Substrates Table provides a comprehensive list of demonstrated downstream targets of Akt phosphorylation.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).
Although publications usually recommend pair-end sequencing for CUT&RUN assay, we find that single-end sequencing works as well. Which one to choose depends on if you would like to know the size of the CUT&RUN DNA fragments, as this information is only provided by pair-end sequencing. If you are only interested in the genomic binding loci of the target protein, but not the length of each DNA fragment by MNase digestion, then single-end sequencing is fine and cost-effective.
Learn how CUT&RUN can be performed in as little as 1-2 days using low cell number samples, and how CUT&RUN has been employed to study epigenetic remodeling and alternative splicing directed by the NF-κB pathway downstream of a viral oncoprotein.
Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.
Expert-reviewed interactive pathway providing a current overview of Regulation of Microtubule Dynamics.
Yes, our DYKDDDDK Tag antibodies will detect 3X FLAG tag as long as the FLAG tag sequence is located on the N-terminal or C-terminal end.
Learn DNA library preparation for ChIP-seq and CUT&RUN using the Illumina® protocol, from end repair & adaptor ligation to PCR enrichment & cleanup.
A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
At AACR 2023, many presentations focused on topics including cancer immunology, CAR-T therapy, spatial biology, mRNA vaccines, and KRAS.
Senescence is a cellular state during which cells remain metabolically active, but irreversibly withdraw from the cell cycle and fail to respond to proliferation-inducing stimuli.
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