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Western Blotting Solutions Kit

Our WB Protocol, Now In a Kit

From sample preparation to detection, everything you need for a more efficient and economical western blot is now found in one easy kit. We get our WB results using these reagents, now you can too.

 
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Pathway Protocol

Changes to Recommended Western Blotting Protocols (Protocols)

A demonstration of the perils of deviating from Cell Signaling Technology's recommended protocols.

Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Protocols)

Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim

Immunoprecipitation Protocol (For Analysis By Kinase Assay) (Protocols)

Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) (Protocols)

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Native Protein) (Protocols)

Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol / (For Denatured Proteins) (IP / Denatured Protein) (Protocols)

Immunoprecipitation of Denatured Proteins Protocol: easy to follow directions describing the step by step experimental procedure.

In-Cell Western Protocol (Protocols)

In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.

Loading Control Antibodies (Product Type Overviews)

Ensure equal sample loading with loading control antibodies from CST.

Product Shelf-Life (Product Shelflife Table)

A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).

Western Blot Reprobing Protocol (Protocols)

Western Blot Reprobing Protocol: easy to follow directions describing the step by step experimental procedure.

Western Blotting & Immunoprecipitation (Application Overviews)

Our antibodies are validated for WB and IP with detailed protocols, control extracts and troubleshooting guides. Download a free white paper and view detailed protocol and troubleshooting videos.

Western Blotting & Immunoprecipitation Technical Support (Application Overviews)

Detailed technical support information to support western blotting experimental success. Link to CST's western blotting protocol.

Western Blotting Protocol (Protocols)

Western Immunoblotting Protocol: easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Fluorescent) (Protocols)

Western Blotting (Fluorescent) Protocol: easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (for Conformation and Light-Chain Specific and Anti-Biotin Secondary Antibodies) (Protocols)

Western Blotting-Conformation, LC Specific, Anti-Biotin Secondary Ab: easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Primary Ab Incubation In BSA) (Protocols)

Western Blotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Primary Ab Incubation In Milk) (Protocols)

Western Blotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol Video

CST's Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final results.

Western Blotting Troubleshooting Guide (Protocols)

Western Blotting troubleshooting guide for easy to solve high and low background issues.

Western Blotting Troubleshooting Guide Video

CST's Western Blotting (WB) Troubleshooting Guide Video provides tips to help you diagnose experimental issues and offers recommendations to solve the problems.

Western Blotting Videos

Short videos from CST highlighting our optimized western blotting protocol and troubleshooting guide to diagnose issues and recommend solutions.

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