SimpleChIP® Enzymatic Chromatin IP Kits from Cell Signaling Technology are optimized to detect endogenous levels of protein-DNA interactions in both cultured cells and SimpleChIP® Plus Chromatin IP Kits offer the additional benefit of tissue sample analysis. These kits contain all reagents necessary to perform enzymatic digestion-based chromatin immunoprecipitation (ChIP) experiments quickly and easily, as well as positive and negative controls that ensure confidence in your results. These kits are available with either Protein G agarose or Protein G magnetic beads and contain all buffers and reagents needed to perform up to 30 ChIP assays.

SimpleChIP® Plus Chromatin IP Kit

Benefits include:

  • Enzymatic digestion of chromatin is milder than sonication and better preserves the integrity of the chromatin and antibody epitopes, which means increased IP efficiency.
  • A detailed and easily scalable protocol optimized for analysis of tissue and cell samples is provided, saving your valuable time and reagents.
  • All reagents and controls are included, saving valuable time and providing the proper experimental controls.
  • These kits are used in-house for validation of our ChIP-recommended antibodies, saving you the trouble of optimization.
  • The included ChIP-grade magnetic beads are ideal for downstream ChIP-on-chip or ChIP-sequencing as they provide higher sensitivity and low background.
  • Technical support is provided by the same scientists who developed and produce the kits, ensuring a thorough, fast, and accurate response.

Mouse Kidney ChIP (Protein G Magnetic Beads)

Mouse Spleen ChIP (Protein G Magnetic Beads)

Figure Caption: Mouse kidney and spleen were cross-linked and disaggregted into single-cell suspensions using a tissue disaggregator, and chromatin was prepared and digested as described in the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. Chromatin immunoprecipitations were performed using the chromatin from mouse kidney (panels A and B) and spleen (panels C and D), and the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Mouse GAPDH Intron 2 Primers #8986, Mouse RPL30 Intron 2 Primers #7015, SimpleChIP® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP® Mouse HoxD10 Intron 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).

SimpleChIP® Plus Enzymatic Chromatin IP Kits

No. Name Applications Reactivity
5571 Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) W, E-P All
9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) ChIP H, M, R, Mk
9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) ChIP H, M, R, Mk
9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) ChIP H, M, R, Mk
9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) ChIP H, M, R, Mk

Applications Key: W=Western blot  ChIP=Chromatin Immunoprecipitation  E-P=ELISA Peptide 
Reactivity Key: H=Human  M=Mouse  R=Rat  Mk=Monkey  All=All Species Expected