These positive and negative control cell extracts and proteins are made and used in-house for antibody validation and lot to lot testing. We recommend using these extracts as control lanes on your western blot, to ensure that western blotting procedures are appropriately executed. The use of proper controls also facilitates troubleshooting, as problems can then be attributed to either the western blotting protocol or the quality of the extracts used in the actual experiment.
For more information, please also see our controls table, which compiles cell lines and treatments that can be used as a positive control for our activation-state specific antibodies and serve as a reference for appropriately controlled experiments.
