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Breast cancer can be driven by several different epigenetic mechanisms. Histone methylation by the Ezh2 protein drives gene silencing required for the maintenance of cell identity. Dysregulation of histone methylation leads to loss of cell identity, which in turn leads to neoplastic transformation and increased breast tumor invasiveness. Additionally, transcriptional repression due to the hypermethylation of nuclear receptor promoter regions leads to the formation of ER– and/or PR– breast cancers that are not responsive to tamoxifen. Finally, phosphorylation of ER at Ser167 promotes ER-dependent transcription and is attributed to increased resistance to tamoxifen

Start with these targets

Ezh2

Overexpression of Ezh2 plays a role in many cancers, including breast cancer, where it adds methyl groups to H3K27 triggering gene silencing.

Products
Ezh2 (D2C9) XP® Rabbit mAb #5246 – WB, IP, IF, F, IHC, ChIP, ChIP-seq
BC Figure 1

Immunohistochemical analysis of paraffin-embedded human lymphoma using Ezh2 (D2C9) XP® Rabbit mAb.

BC Figure 2

Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA for EZH2 ChIP-seq and 50ng enriched ChIP DNA for H3K27me3 ChIP-seq using SimpleChIP® ChIP-Seq DNA Library Prep Kit for Illumina® #56795, and sequenced on the Illumina NextSeq. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across the MYT1 gene.

UTX

UTX is a counterpart to Ezh2 that removes methyl groups from H3K27.

Products
UTX (D3Q1I) Rabbit mAb #33510 – WB, IP, IHC
BC Figure 3

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using UTX (D3Q1I) Rabbit mAb.

Estrogen Receptor α and Progesterone Receptor

Mutations in nuclear receptors such as Estrogen Receptor α and Progesterone Receptor will alter gene expression and can have a huge impact on treatment options in breast cancer.

H3K27me3

Watch out for increased H3K27 methylation caused by Ezh2 overexpression or UTX mutation.