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Prostate Cancer

Androgen receptor (AR) signaling is the central signal transduction pathway in prostate cancer. Many different epigenetic mechanisms affecting this critical pathway play a role in disease initiation and progression. This includes the presence of AR variants that are constitutively transcriptionally active, interference with histone demethylase coactivators, and the formation of gene fusions of AR-responsive genes with transcription factors like ERG.

Start with these targets

Androgen Receptor

Androgen Receptor (AR) transcripts can include cryptic exons, which result in early termination of translation and proteins lacking in the ligand binding domain. AR-V7, a variant arising from a cryptic exon, leads to aberrant transcriptional regulation and is frequently expressed in castration-resistant prostate cancer (CRPC).

Products
Androgen Receptor (D6F11) XP® Rabbit mAb #5153 – WB, IP, IF, F, IHC, ChIP
PC Figure 1

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Androgen Receptor (D6F11) XP® Rabbit mAb.

Glucocorticoid Receptor

In some prostate cancers, Glucocorticoid Receptor (GR) signaling may aberrantly override expression of AR targets, inducing further cancer progression and AR-treatment resistance. In these instances, inhibition of Ezh2, which can act as a coactivator for Androgen Receptor, offers additional treatment options.

Products
Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 – WB, IP, IF, F, IHC, ChIP, ChIP-seq
PC Figure 2

Chromatin immunoprecipitations were performed with cross-linked chromatin from A549 cells cultured in media with 5% charcoal-stripped FBS for 3 d and then treated with 100 nM dexamethasone for 1 hr and Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using SimpleChIP® ChIP-Seq DNA Library Prep Kit for Illumina® #56795, and sequenced on the Illumina NextSeq. The figure shows binding across SLC19A2, a known target gene of GR (see additional figure containing ChIP-qPCR data).

Ezh2 (D2C9) XP® Rabbit mAb #5246 – W, IP, IF, F, IHC, ChIP, ChIP-seq
HOXB13

HOXB13 is a transcription factor and marker of prostate cancer that is used to identify metastasis.

Products
HOXB13 (D7N8O) Rabbit mAb #90944 – W, IP, IHC
ERG

ERG overexpression occurs due to gene fusion with the androgen-driven promoter of the TMPRSS2 gene and is a key driver of prostate cancer metastasis.

Products
ERG (A7L1G) Rabbit mAb #97249 – W, IP, F, IHC
PC Figure 3

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma (two different cases) using ERG (A7L1G) Rabbit mAb.