Buy Three, Get the Fourth Free! | Start Saving >>

Chromatin Immunoprecipitation Workflow Solutions for ChIP-qPCR and ChIP-seq

Every reagent you need for ChIP. All in one place. All from CST.

Add a whole new level of convenience to your ChIP experiments.

CST’s new additions to the SimpleChIP® product lineup give you complete workflow solutions for ChIP-qPCR and ChIP-seq experiments with no-hassle, one-stop shopping for everything you need.

Our SimpleChIP-qPCR Master Mix, DNA Library Prep Kit, and Multiplex Oligos come with ChIP-specific protocols that make it even easier to perform your entire ChIP experiment.

We use the same reagents and kits to validate our own ChIP and ChIP-seq antibodies.

SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 — Minimize loss of input DNA and generate the highest library yields, even with limited amounts of DNA. The kit comes with all the enzymes and buffers you need to generate high-quality DNA sequencing libraries from ChIP DNA for NG-seq on the Illumina platform. The fast, user-friendly workflow also cuts down on the hands-on time needed for generating and purifying DNA libraries. Learn more.

SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single #29580 or Dual Index Primers #47538) — Our adaptors and primers are ideally suited for multiplex sample preparation with the SimpleChIP ChIP-seq DNA Library Prep Kit for NG-seq on the Illumina platform. Learn more.

SimpleChIP® Universal qPCR Master Mix #88989 — Get rapid, sensitive, and precise dye-based qPCR detection and quantitation of target DNA sequences. Our new master mix matches the performance of the market leader. You can also stick with the same SYBR/FAM channel you’re currently using with most real-time qPCR instruments. Just in case you need them, we also have over 100 primers to choose from. Learn more.

SimpleChIP® Universal qPCR Master Mix provides increased sensitivity and linearity when compared to the market leader.

Universal qPCR Master Mix provides increased sensitivity

Amplification plot of a serial dilution of input DNA samples. Input DNA was prepared and purified from Hela cells using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. Quantitative PCR was performed with 20 (red line), 4 (orange line), 0.8 (purple line), and 0.016 (blue line) ng of input DNA and either SimpleChIP® Human α Satellite Repeat Primers #4486 (left two panels) or SimpleChIP®Human RPL30 Exon 3 Primers #7014 (right two panels), using SimpleChIP® Universal qPCR Master Mix #88989 (upper two panels) or Market Leader (lower two panels). Data is shown for two replicates.

SimpleChIP® Universal qPCR Master Mix shows equivalent amplification efficiency across a wide range of input DNA amounts as compared to the market leader.

Universal qPCR Master Mix shows equivalent amplificatio

Regression curve of a serial dilution of input DNA samples. Input DNA was prepared and purified from Hela cells using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. Quantitative PCR was performed with 20, 4, 0.8, and 0.016 ng of input DNA and either #4486 SimpleChIP® Human α Satellite Repeat Primers (left two panels) or SimpleChIP® Human RPL30 Exon 3 Primers #7014 (right two panels), using SimpleChIP® Universal qPCR Master Mix #88989 (upper two panels) or Market Leader (lower two panels). Data is shown for two replicates.

SimpleChIP® Universal qPCR Master Mix provides identical specificity as the market leader, as shown by PCR product melt curves

ChIP workflow using Universal qPCR Master Mix

Corresponding single melt peak of PCR products. Quantitative PCR was performed with input DNA sample from HCT 116 cells prepared by SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 and either SimpleChIP® Human CaMK2D Intron 3 Primers #5111 (purple line), SimpleChIP® Human α Satellite Repeat Primers #4486 (red line), SimpleChIP®Human RPL30 Exon 3 Primers #7014 (orange line), or human c-Myc promoter primers (blue line), using SimpleChIP® Universal qPCR Master Mix #88989 (left) or Market Leader (right). Data for two replicates is shown for each primer set.

Equivalent ChIP-qPCR results are achieved using SimpleChIP® Universal qPCR Master Mix when compared to market leader.

ChIP Workflow using ChIP qPCR

Quantification of ChIP DNA enriched using Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, TCF4/TCF7L2 (C48H11) Rabbit mAb #2569, and Normal Rabbit IgG #2729 by SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 (blue and red bars; left panel), TCF4/TCF7L2 (C48H11) Rabbit mAb #2569 (blue and red bars; right panel) or Normal Rabbit IgG #2729 (green and purple bars; both panels). The enriched DNA was quantified by real-time PCR with either SimpleChIP® Human RPL30 Exon 3 Primers #7014, human c-Myc promoter primers, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, or SimpleChIP® Human α Satellite Repeat Primers #4486, using either Market Leader (blue and green bars) or SimpleChIP® Universal qPCR Master Mix #88989 (red and purple bars). The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Illumina is a registered trademark of Illumina, Inc.

SimpleChIP® Universal qPCR Master Mix contains NEB® Luna® qPCR reagent

SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® contains NEBNext® Ultra™ II DNA reagents

SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index and Dual Index Primers) contains NEBNext® Oligos