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ELISA Solutions

Cell Signaling Technology has a wide selection of sandwich antibody assays that let you measure important cellular signaling nodes and other cellular target proteins. In-house CST scientists identify optimal antibody pairs, develop, produce, and thoroughly validate our ELISA products to ensure the highest possible product quality, sensitivity, data consistency, and accuracy. Customer technical support is provided by the same scientists who develop our ELISA products.

FastScan Lab Process

ELISA Product Line

  • FastScan™ ELISA kits – include all the items you need to measure key cellular signaling proteins. Simple protocols and a less complex, solution-based assay format lets you detect targets in as little as 90 minutes, much faster than conventional protocols. Kits are available for both phosphorylation-specific and total protein options and come with integrated controls.
  • PathScan® Sandwich ELISA kits – include all the items you need to detect endogenous levels of key signaling molecules using a traditional, sandwich-based assay format. Matched phospho and total protein kits are available for many targets, as well as chemiluminescent and colorimetric detection options.
  • PathScan® Sandwich ELISA Antibody Pairs – provide an economical alternative to our complete ELISA kits, formulated for traditional, sandwich-based assay formats.

Which CST ELISA Solution is Best for Me?

Choosing the right ELISA solution is important, and we want you to get the absolute best results for your research project. Here’s a quick comparison of our FastScan and PathScan ELISA kits and assays to help you decide.

  FastScan ELISA Kits PathScan ELISA Kits and ELISA Pairs
Assay type Solution-based sandwich Solid-phase sandwich
Hands-on time 15 min Kits (pre-coated plates):
25 min
Pairs (plates coated by user):
>45 min
Number of wash steps 1 Many
Typical time to result 90 mins Kits (pre-coated plates):
240 min
Pairs: (plates coated by user):
>240 min - overnight
ELISA experience level Beginner-Advanced Beginner-Advanced
Sensitivity Comparable or better than traditional ELISA Comparable to traditional ELISA
Dynamic range Comparable to traditional ELISA Comparable to traditional ELISA
Sample types Cell extract/tissue extract/other1 Cell extract/tissue extract/other1
Internal control for assay performance included in kit Yes No
Quantitation Semi-quantitative Semi-quantitative
Detection Colorimetric Colorimetric – All
Chemiluminescence – Available with specific kits
Types of targets Post-translational modifications, endogenous cellular proteins Post-translational modifications, endogenous cellular proteins

1 Other sample types may be compatible depending on specific kit. Samples must be prepared in such a way that they are compatible with immune reactivity.

PathScan (left) and FastScan (right) ELISA assay methods.

Figure: Comparison of Pathscan (left) and FastScan (right) ELISA assay methods.

In the Pathscan ELISA assay method the capture antibody is pre-adsorbed onto the microplate well in advance. Sample, Detection Ab and a secondary HRP-linked Ab are each added serially with incubation and multi-wash steps in between each addition. The capture antibody binds to one site of the sample molecule, where the detection antibody recognizes another site, thereby forming the sandwich around the target.

FastScan ELISA kits use a streamlined format wherein the antibody-target sandwich complex is formed in solution in a single incubation, requiring only one wash. The capture antibody, detection antibody and target sample are mixed and added to a microplate well. The entire complex is anchored to the well via an anti-tag antibody, which has been pre-adsorbed in the well.

Customize Your Throughput and Packaging

Our ELISA kits are also available in bulk quantities with a choice of either 96- or 384-well plates so you can customize them just for your lab. If you need a 384-well format or bulk packaging of 5 or more kits, please contact CST's sales department for processing time and pricing.