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Chromatin Crosslinking: How Much Time? Chromatin Immunoprecipitation (ChIP)

 

Crosslinking - or fixation - is the first step in the ChIP protocol. The purpose of crosslinking is to prevent dissociation of DNA from the protein during the 3-day ChIP experiment, and allow the antibody to pull down the DNA. Learn how the optimal crosslinking time in Chromatin Immunoprecipitation (ChIP) can be influenced by sample type, and by target protein (histone, transcription factor, or cofactor) in this Tech Tip video./p>