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How to Choose the Appropriate ChIP Protocol and Generate Reliable Data

 

Chromatin fragmentation is a critical step in the ChIP protocol and can affect the quality of your data. Different target types (histone modifications, DNA-binding transcription factors, and transcription cofactors) and different sample types each present their own challenges. This presentation outlines considerations for choosing between enzymatic or sonication approaches, along with advice for troubleshooting ChIP-qPCR results and QC of ChIP-seq libraries.

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