Chromatin fragmentation can be performed using either sonication or enzymatic digestion. Despite its name, the latter still requires a brief sonication step to break open nuclear membranes and release the chromatin after digestion with micrococcal nuclease. Watch this Tech Tip video to learn how to tell if your sample is ready for DNA purification and subsequent steps, or if it requires further sonication first./p>
Using the Sonication ChIP Protocol? Watch the companion video.