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Milk or BSA? Choosing a Blocking Protein for Western Blotting (WB)


How does choice of blocking protein - nonfat dry milk vs bovine serum albumen (BSA) - affect your Western Blot (WB) results? The purpose of the blocking step is to reduce the amount of background on the membrane caused by nonspecific binding of primary or secondary antibody. In this video, Srikanth explains the logic behind our protocol recommendation for using diluted nonfat dry milk, even for phospho-specific proteins.