| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Shh (C9C5) Rabbit mAb | 2207 | 20 µl | 19, (45 precursor) kDa | Rabbit IgG |
| Smoothened (E6Z5T) Rabbit mAb | 92981 | 20 µl | 85 kDa | Rabbit IgG |
| PTCH1 (C53A3) Rabbit mAb | 2468 | 20 µl | 180-210 kDa | Rabbit IgG |
| PTCH2 (G1191) Antibody | 2470 | 20 µl | 130 kDa | Rabbit |
| SUFU (C54G2) Rabbit mAb | 2520 | 20 µl | 54 kDa | Rabbit IgG |
| GLI1 (C68H3) Rabbit mAb | 3538 | 20 µl | 160 kDa | Rabbit IgG |
| GLI2 (E5V8N) Rabbit mAb | 18773 | 20 µl | 220 kDa | Rabbit IgG |
| GLI3 (E6E2K) Rabbit mAb | 71107 | 20 µl | 200 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Hedgehog Signaling Antibody Sampler Kit provides an economical means of evaluating key members of the Hedgehog signaling pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The Hedgehog (Hh) signaling pathway plays critical roles in the regulation of cell fate, tissue patterning, and growth during embryonic development. It is downregulated during postnatal development, but can be reactivated to promote tissue repair and regeneration. Aberrant Hh signaling activity during prenatal development is associated with numerous birth defects (e.g., holoprosencephaly), while uncontrolled Hh pathway activation postnatally is linked to the development of several cancer types (1,2). There are three canonical Hh ligands: Sonic hedgehog (SHH), Indian hedgehog (IHH), and Desert hedgehog (DHH), all of which have distinct as well as overlapping roles and expression patterns (3-5). Patched1 and 2 (PTCH1 and PTCH2) are partially redundant 12-pass transmembrane proteins that function as receptors for Hh ligands (6-8). Smoothened (SMO) is a 7-pass transmembrane G protein-coupled receptor (GPCR) that functions as the key transducer of Hh signaling. In the absence of Hh ligands (off-state), PTCH proteins are localized to cilia, and function to suppress SMO activity (1,2). Suppressor of Fused (SUFU) simultaneously contributes to suppression of the pathway by sequestering the glioma-associated oncogene (GLI) family of transcription factors (9,10). Binding of Hh ligands to PTCH receptors results in derepression of SMO, in part by promoting its translocation to cilia; this leads to downregulation of SUFU, resulting in the stabilization and nuclear translocation of GLI transcription factors that regulate the transcription of genes involved in cell proliferation, migration, and survival (1).
- Lee, R.T. et al. (2016) Development 143, 367-72.
- McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
- Zhang, X.M. et al. (2001) Cell 106, 781-92.
- Adolphe, C. et al. (2004) Development 131, 5009-19.
- Pathi, S. et al. (2001) Mech Dev 106, 107-17.
- Stone, D.M. et al. (1996) Nature 384, 129-34.
- Motoyama, J. et al. (1998) Nat Genet 18, 104-6.
- Smyth, I. et al. (1999) Hum Mol Genet 8, 291-7.
- Barnfield, P.C. et al. (2005) Differentiation 73, 397-405.
- Méthot, N. and Basler, K. (2000) Development 127, 4001-10.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.