Revision 1

#33738Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Complexin-1 (D5Q5H) Rabbit mAb 17700 20 µl 14 kDa Rabbit IgG
Complexin-1/2 (D8A6E) Rabbit mAb 28070 20 µl 14-16 kDa Rabbit IgG
Munc18-1 (D4O6V) Rabbit mAb 13414 20 µl 70 kDa Rabbit IgG
SNAP25 (D7B4) Rabbit mAb 5308 20 µl 25 kDa Rabbit IgG
Synaptophysin (D8F6H) XP® Rabbit mAb 36406 20 µl 38 kDa Rabbit IgG
Synaptotagmin-1 (D33B7) Rabbit mAb 14558 20 µl 60 kDa Rabbit IgG
Synapsin-1 (D12G5) XP® Rabbit mAb 5297 20 µl 77 kDa Rabbit IgG
Syntaxin 1A (D4E2W) Rabbit mAb 18572 20 µl 33 kDa Rabbit IgG
VAMP2 (D6O1A) Rabbit mAb 13508 20 µl 13 kDa Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Presynaptic Vesicle Cycle Antibody Sampler Kit provides an economical means of detecting expression of key synaptic vesicle proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


The synapse is the unit of information transfer in the brain between neurons (1). Synaptic transfer is initiated by action potential-initiated synaptic vesicles fusion at the presynaptic terminal to release neurotransmitters. The basic molecular machinery that drives all membrane fusion events in all cells include N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that form a trans-ternary complex between to-be-fused membranes. The zippering up of SNARE proteins provides the energy required for membrane fusion. At the synapse, the SNARE proteins include 25 kDa synaptosome-associated protein (SNAP25), syntaxin 1A (STX1A), and vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin). Neurotransmitter release, however, is a highly regulated process that occurs in response to action potential-initiated Ca2+-influx. Regulation of synaptic vesicle fusion is mediated by SNARE-binding proteins like complexin and the Ca2+-associated vesicle protein, synaptotagmin-1, that cooperatively function to initiate Ca2+-induced synaptic vesicle fusion. The synaptic vesicle cycle represents a cycle of events that prime the synaptic vesicle before fusion and retrieve fused membrane after full fusion. Synaptic vesicle-associated proteins like synaptophysin and synapsin-1, as well as target membrane proteins like Munc18-1, play a complex role in priming synaptic vesicles to be fusion competent.   

  1. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474-7.

Background References

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