| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| MyD88 (D80F5) Rabbit mAb | 4283 | 20 µl | 33 kDa | Rabbit IgG |
| IRAK1 (D51G7) Rabbit mAb | 4504 | 20 µl | 78-105 kDa | Rabbit IgG |
| IRAK2 Antibody | 4367 | 20 µl | 62 kDa | Rabbit |
| IRAK4 Antibody | 4363 | 20 µl | 55 kDa | Rabbit |
| Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb | 11927 | 20 µl | 55 kDa | Rabbit IgG |
| TRAF6 (E2K9D) Rabbit mAb | 67591 | 20 µl | 60 kDa | Rabbit IgG |
| TBK1/NAK (E8I3G) Rabbit mAb | 38066 | 20 µl | 84 kDa | Rabbit IgG |
| Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb | 5483 | 20 µl | 84 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Myddosome Complex Antibody Sampler Kit provides an economical means of detecting the components of the myddosome complex using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Toll-like receptors (TLRs) are a large family of so-called pattern recognition receptors (PRRs) that detect pathogen associated molecular patterns (PAMPs) and danger associated molecular patterns (DAMPs) (1,2). Upon activation, TLRs initiate two main signaling pathways through their C-terminal cytoplasmic Toll/Il-1 receptor (TIR) domain that couples with TIR domain-containing adaptors MyD88 and TRIF. The MyD88-dependent pathway is initiated by the formation of a large oligomeric protein complex termed myddosome. Myddosome is one of so-called supramolecular organizing centers (SMOCs), a signaling organelle that is common for PRRs in the innate immune system. Myddosome formation promotes IRAK4 activation, which in turn activates IRAK1 and later, IRAK2. TRAF6 is then recruited and activated through the binding sites within IRAKs. Activated TRAF6 is released to the cytosol and triggers the IKK complex to activate the NF-κB pathway to mediate the expression of pro-inflammatory cytokines and chemokines (3-7). Recently, it was also found that TBK1 is recruited to the myddosome complex and activated to induce aerobic glycolysis (8).
- Behzadi, P. et al. (2021) J Immunol Res 2021, 9914854.
- Aluri, J. et al. (2021) Cells 10, 1374. doi: 10.3390/cells10061374.
- De Nardo, D. (2015) Cytokine 74, 181-9.
- Latty, S.L. et al. (2018) Elife 7:e31377. doi: 10.7554/eLife.31377.
- De Nardo, D. et al. (2018) J Biol Chem 293, 15195-15207.
- Balka, K.R. and De Nardo, D. (2019) J Leukoc Biol 105, 339-351.
- Gold, J.J. et al. (1988) Biosystems 21, 403-15.
- Tan, Y. and Kagan, J.C. (2019) Cell 177, 384-398.e11.
Background References
Trademarks and Patents
Limited Uses
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