Revision 2

#42521Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
LRP1 (E2Q7S) Rabbit mAb 26387 20 µl 85 kDa Rabbit IgG
ApoE (pan) (D7I9N) Rabbit mAb 13366 20 µl 35 kDa Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl 50-80 kDa Rabbit IgG
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb 12885 20 µl 50-80 kDa Rabbit IgG
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb 20194 20 µl 50-80 kDa Rabbit IgG
SORL1 (D8D4G) Rabbit mAb 79322 20 µl 250 kDa Rabbit IgG
Rab5 (C8B1) Rabbit mAb 3547 20 µl 25 kDa Rabbit 
Rab7 (D95F2) XP® Rabbit mAb 9367 20 µl 23 kDa Rabbit IgG
Rab11 (D4F5) XP® Rabbit mAb 5589 20 µl 25 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit provides an economical means of detecting components of the LRP-1 mediated intercellular transmission of human tau using antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. In addition to its normal function, intracellular neurofibrillary tangle protein aggregates, composed of hyperphosphorylated helical bundles of tau, are a major hallmark of neurodegenerative diseases like Alzheimer's disease (AD) (1). Moreover, disease progression is also measured by the progressive spread and deposition of the protein aggregates via intercellular transfer of tau (2). Although the intercellular mechanism of protein aggregate transfer is poorly understood, low density lipoprotein receptor related protein 1 (LRP1) was identified as a regulator of tau uptake and spread (3). LRP1 is a type I transmembrane receptor that mediates the endocytosis of various ligands, including apolipoproteins and tau. Interestingly, human apolipoprotein E (ApoE), which also binds to LRP1, is genetically linked to AD (4). LRP1-mediated protein uptake, in addition to tau, may play an important role in AD progression. In addition to LRP1, other low density lipoprotein receptor related proteins, including SORL1, are genetically linked to AD, suggesting a conserved cellular mechanism that converges on this family of proteins that contributes to AD etiology (5). Once tau binds to LRP1, receiving cells are likely to internalize and process tau via the endosomal pathway, completing cell-to-cell transmission. Rab5, Rab7, and Rab11, members of the Ras superfamily of small Rab GTPases, are likely to regulate endosomal processing of tau (6). 

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Braak, H. and Braak, E. (1991) Acta Neuropathol 82, 239-59.
  3. Rauch, J.N. et al. (2020) Nature 580, 381-385.
  4. Corder, E.H. et al. (1993) Science 261, 921-3.
  5. Yin, R.H. et al. (2015) Mol Neurobiol 51, 909-18.
  6. Zhang, X. et al. (2019) ACS Chem Neurosci 10, 828-838.

Background References

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    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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