Revision 2

#42521Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
LRP1 (E2Q7S) Rabbit mAb 26387 20 µl 85 kDa Rabbit IgG
ApoE (pan) (D7I9N) Rabbit mAb 13366 20 µl 35 kDa Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl 50-80 kDa Rabbit IgG
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb 12885 20 µl 50-80 kDa Rabbit IgG
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb 20194 20 µl 50-80 kDa Rabbit IgG
SORL1 (D8D4G) Rabbit mAb 79322 20 µl 250 kDa Rabbit IgG
Rab5 (C8B1) Rabbit mAb 3547 20 µl 25 kDa Rabbit 
Rab7 (D95F2) XP® Rabbit mAb 9367 20 µl 23 kDa Rabbit IgG
Rab11 (D4F5) XP® Rabbit mAb 5589 20 µl 25 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit provides an economical means of detecting components of the LRP-1 mediated intercellular transmission of human tau using antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. In addition to its normal function, intracellular neurofibrillary tangle protein aggregates, composed of hyperphosphorylated helical bundles of tau, are a major hallmark of neurodegenerative diseases like Alzheimer's disease (AD) (1). Moreover, disease progression is also measured by the progressive spread and deposition of the protein aggregates via intercellular transfer of tau (2). Although the intercellular mechanism of protein aggregate transfer is poorly understood, low density lipoprotein receptor related protein 1 (LRP1) was identified as a regulator of tau uptake and spread (3). LRP1 is a type I transmembrane receptor that mediates the endocytosis of various ligands, including apolipoproteins and tau. Interestingly, human apolipoprotein E (ApoE), which also binds to LRP1, is genetically linked to AD (4). LRP1-mediated protein uptake, in addition to tau, may play an important role in AD progression. In addition to LRP1, other low density lipoprotein receptor related proteins, including SORL1, are genetically linked to AD, suggesting a conserved cellular mechanism that converges on this family of proteins that contributes to AD etiology (5). Once tau binds to LRP1, receiving cells are likely to internalize and process tau via the endosomal pathway, completing cell-to-cell transmission. Rab5, Rab7, and Rab11, members of the Ras superfamily of small Rab GTPases, are likely to regulate endosomal processing of tau (6). 

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Braak, H. and Braak, E. (1991) Acta Neuropathol 82, 239-59.
  3. Rauch, J.N. et al. (2020) Nature 580, 381-385.
  4. Corder, E.H. et al. (1993) Science 261, 921-3.
  5. Yin, R.H. et al. (2015) Mol Neurobiol 51, 909-18.
  6. Zhang, X. et al. (2019) ACS Chem Neurosci 10, 828-838.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.