| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-IRF-3 (Ser386) (E7J8G) XP® Rabbit mAb | 37829 | 20 µl | 50-55 kDa | Rabbit IgG |
| IRF-3 (D6I4C) XP® Rabbit mAb | 11904 | 20 µl | 50-55 kDa | Rabbit IgG |
| Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb | 12390 | 20 µl | 65 kDa | Rabbit IgG |
| IRF-7 (D2A1J) Rabbit mAb | 13014 | 20 µl | 65 kDa | Rabbit IgG |
| IFN-β1 (D1D7G) Rabbit mAb | 73671 | 20 µl | 19, 21 kDa | Rabbit IgG |
| Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb | 8826 | 20 µl | 91 kDa | Rabbit IgG |
| Stat1 (D1K9Y) Rabbit mAb | 14994 | 20 µl | 84, 91 kDa | Rabbit IgG |
| IRF-9 (D2T8M) Rabbit mAb | 76684 | 20 µl | 48 kDa | Rabbit IgG |
| MX1 (D3W7I) Rabbit mAb | 37849 | 20 µl | 76 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Type I Interferon Induction and Signaling Antibody Sampler Kit provides an economical means of detecting the activation of pathways leading to upregulation of type I interferon (IFN), expression of IFN-β1, activation of signaling downstream of type I IFN, and expression of the MX1 interferon response gene, using phospho-specific and control antibodies. The kit includes enough antibodies to perform at least two western blot experiments.
Storage
Background
The innate immune system uses pattern recognition receptors (PRRs) that detect conserved pathogen-associated molecular patterns (PAMPs), such as cytoplasmic double-stranded RNA, to detect and initiate an immune response to viral infection. Detection of virus by PRRs leads to phosphorylation and nuclear translocation of IRF-3 and IRF-7, resulting in upregulation of type I interferons, which include IFN-α and IFN-β (1-3). Type I interferons signal through the interferon α/β receptor (IFNAR), leading to phosphorylation and activation of Stat1 and Stat2, which form a complex with IRF-9 (4,5). This complex translocates to the nucleus where it induces transcription of interferon response genes including viral restriction factors, such as MX1, that limit viral replication and propagation (4-7).
- Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.
- Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
- Sato, M. et al. (2000) Immunity 13, 539-48.
- Fu, X.Y. et al. (1990) Proc Natl Acad Sci U S A 87, 8555-9.
- Qureshi, S.A. et al. (1995) Proc Natl Acad Sci U S A 92, 3829-33.
- Staeheli, P. et al. (1986) Cell 44, 147-58.
- Staeheli, P. and Haller, O. (1985) Mol Cell Biol 5, 2150-3.
Background References
Trademarks and Patents
Limited Uses
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