Revision 1

#4763Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
eIF4A (C32B4) Rabbit mAb 2013 20 µl 48 kDa Rabbit IgG
eIF4A1 Antibody 2490 20 µl 48 kDa Rabbit 
Phospho-eIF4B (Ser422) Antibody 3591 20 µl 80 kDa Rabbit 
eIF4B Antibody 3592 20 µl 80 kDa Rabbit 
Phospho-eIF4E (Ser209) Antibody 9741 20 µl 25 kDa Rabbit 
eIF4E (C46H6) Rabbit mAb 2067 20 µl 25 kDa Rabbit IgG
Phospho-eIF4G (Ser1108) Antibody 2441 20 µl 220 kDa Rabbit 
eIF4G (C45A4) Rabbit mAb 2469 20 µl 220 kDa Rabbit 
eIF4H (D85F2) XP® Rabbit mAb 3469 20 µl 25, 27 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Translation Initiation Complex Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).

  1. Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608.
  2. Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
  3. Svitkin, Y.V. et al. (2001) RNA 7, 382-94.
  4. Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
  5. Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
  6. Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit for more information.

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