| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| eIF4A (C32B4) Rabbit mAb | 2013 | 20 µl | 48 kDa | Rabbit IgG |
| eIF4A1 Antibody | 2490 | 20 µl | 48 kDa | Rabbit |
| Phospho-eIF4B (Ser422) Antibody | 3591 | 20 µl | 80 kDa | Rabbit |
| eIF4B Antibody | 3592 | 20 µl | 80 kDa | Rabbit |
| Phospho-eIF4E (Ser209) Antibody | 9741 | 20 µl | 25 kDa | Rabbit |
| eIF4E (C46H6) Rabbit mAb | 2067 | 20 µl | 25 kDa | Rabbit IgG |
| Phospho-eIF4G (Ser1108) Antibody | 2441 | 20 µl | 220 kDa | Rabbit |
| eIF4G (C45A4) Rabbit mAb | 2469 | 20 µl | 220 kDa | Rabbit |
| eIF4H (D85F2) XP® Rabbit mAb | 3469 | 20 µl | 25, 27 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Translation Initiation Complex Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.
Storage
Background
A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).
- Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608.
- Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-94.
- Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
- Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
- Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
Background References
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.