Revision 7

#56511Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Phospho-Tau (Thr205) (E7D3E) Rabbit mAb 49561 20 µl 50-80 kDa Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl 50-80 kDa Rabbit IgG
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl 46-55 kDa Rabbit IgG
Synaptophysin (7H12) Mouse mAb (IF Formulated) 9020 20 µl Mouse IgG1
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl 95 kDa Rabbit IgG
Cleaved Caspase-3 (Asp175) Antibody 9661 20 µl 17, 19 kDa Rabbit 
Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 94885 20 µl 89 kDa Rabbit IgG
GFAP (E6N9L) Mouse mAb 34001 20 µl 50 kDa Mouse IgG2a
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl 80 kDa Rabbit IgG

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Tau Mouse Model Neuronal Viability IF Antibody Sampler Kit provides an economical means of detecting proteins to confirm neuronal viability and surrounding astrocytes and microglia in mouse models by immunofluorescence.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Neurofibrillary tangles are a major pathological hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau, including phosphorylation of tau at Thr205 (1,2). Research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (4). Glial fibrillary acidic protein (GFAP) is the main intermediate filament in mature brain astroglial and radial glial cells. GFAP plays an important role in modulating astroglial motility and shape (5). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (6). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 to be a useful and specific tool to study microglia (7). Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that occurs in presynaptic vesicles of neurons (8). Postsynaptic density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (9,10). Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, including nuclear enzyme poly (ADP-ribose) polymerase (PARP) (11). PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (12). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (13).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Wang, J. et al. (2000) Zhongguo Yi Xue Ke Xue Yuan Xue Bao 22, 120-3.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Mullen, R.J. et al. (1992) Development 116, 201-11.
  5. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  6. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  7. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  8. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci U S A 83, 3500-4.
  9. Cao, J. et al. (2005) J Cell Biol 168, 117-26.
  10. Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
  11. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  12. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  13. Oliver, F.J. et al. (1998) J Biol Chem 273, 33533-9.

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