Revision 1

#59633Store at -20C

1 Kit

(7 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
AS160 (C69A7) Rabbit mAb 2670 20 µl 160 kDa Rabbit 
Phospho-AS160 (Ser318) (D3D11) Rabbit mAb 8619 20 µl 160 kDa Rabbit IgG
Phospho-AS160 (Ser588) (D8E4) Rabbit mAb 8730 20 µl 160 kDa Rabbit IgG
Phospho-AS160 (Thr642) (D27E6) Rabbit mAb 8881 20 µl 160 kDa Rabbit IgG
Akt (pan) (C67E7) Rabbit mAb 4691 20 µl 60 kDa Rabbit IgG
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb 13038 20 µl 60 kDa Rabbit IgG
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 20 µl 60 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The AS160 Signaling Antibody Sampler Kit provides an economical means of detecting select components involved in the AS160 signaling pathway. The kit contains enough primary antibodies to perform at least two western blot experiments per antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


Insulin is a major hormone controlling critical energy functions, such as glucose and lipid metabolism. Insulin binds to and activates the insulin receptor (IR) tyrosine kinase, which phosphorylates and recruits adaptor proteins. The signaling pathway initiated by insulin and its receptor stimulates glucose uptake in muscle cells and adipocytes through translocation of the Glut4 glucose transporter from the cytoplasm to the plasma membrane (1). A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regulates insulin-stimulated Glut4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat (2). Multiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insulin treatment (2,3). Studies using recombinant AS160 demonstrate that insulin-stimulated phosphorylation of AS160 is a crucial step in Glut4 translocation (3) and is reduced in some patients with type 2 diabetes (4). The interaction of 14-3-3 regulatory proteins with AS160 phosphorylated at Thr642 is a necessary step for Glut4 translocation (5). Phosphorylation of AS160 by AMPK is involved in the regulation of contraction-stimulated Glut4 translocation (6).
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (7-9). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (8,9). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (10) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as the mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (11,12).

  1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-22.
  2. Kane, S. et al. (2002) J. Biol. Chem. 277, 22115-8.
  3. Sano, H. et al. (2003) J. Biol. Chem. 278, 14599-602.
  4. Karlsson, H.K. et al. (2005) Diabetes 54, 1692-7.
  5. Ramm, G. et al. (2006) J. Biol. Chem. 281, 29174-80.
  6. Kramer, H.F. et al. (2006) J. Biol. Chem. 281, 31478-85.
  7. Franke, T.F. et al. (1997) Cell 88, 435-7.
  8. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
  9. Franke, T.F. et al. (1995) Cell 81, 727-36.
  10. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  11. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  12. Jacinto, E. et al. (2006) Cell 127, 125-37.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit for more information.

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