Revision 5

#70076Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IHC-P

REACTIVITY:

M

SENSITIVITY:

Endogenous

MW (kDa):

65-250

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q61549

Entrez-Gene Id:

13733

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:125 - 1:500

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #25514.

Specificity / Sensitivity

F4/80 (D2S9R) XP® Rabbit mAb recognizes endogenous levels of total F4/80 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant mouse F4/80 protein.

Background

F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4).

  1. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  2. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  3. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  4. Greter, M. et al. (2015) Front Immunol 6, 249.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 5
#70076

F4/80 (D2S9R) XP® Rabbit mAb

Western Blotting Image 1: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation Image 1: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Immunoprecipitation of F4/80 from BaF3 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is F4/80 (D2S9R) XP® Rabbit mAb, #70076. Western blot was performed using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemistry Image 1: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemistry Image 2: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemistry Image 3: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemistry Image 4: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb.
Immunohistochemistry Image 5: F4/80 (D2S9R) XP® Rabbit mAb Expand Image
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using F4/80 (D2S9R) XP® rabbit mAb (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan).