Revision 1

#78551Store at -20C

1 Kit

(6 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
p16 INK4A (E5F3Y) Rabbit mAb 29271 20 µl 16 kDa Rabbit IgG
p21 Waf1/Cip1 (E2R7A) Rabbit mAb 37543 20 µl 21 kDa Rabbit IgG
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb 9718 20 µl 15 kDa Rabbit IgG
Lamin B1 (E6M5T) Rabbit mAb 17416 20 µl 68, 45 kDa Rabbit IgG
HMGB1 (D3E5) Rabbit mAb 6893 20 µl 29 kDa Rabbit IgG
IL-6 (D5W4V) XP® Rabbit mAb 12912 20 µl 24 kDa Rabbit IgG
TNF-α (D2D4) XP® Rabbit mAb 11948 20 µl 17,25,28 kDa Rabbit IgG
MMP-2 (D2O4T) Rabbit mAb 87809 20 µl 64,72 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Mouse Reactive Senescence Marker Antibody Sampler Kit provides an economical means of detecting multiple markers of cellular senescence in mouse samples. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors, and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2).
Because there is no single biomarker that can be used to definitively identify senescent cells, researchers must rely on a collection of biomarkers commonly associated with senescence (3). The Mouse Reactive Senescence Marker Antibody Sampler Kit provides a collection of antibodies to commonly used biomarkers of senescence-associated cell cycle arrest (p16 INK4A, p21 Waf1/Cip1), senescence-associated DNA damage (gamma-Histone H2A.X), and the SASP (HMGB1, IL-6, TNF-α, MMP2). The kit also includes an antibody to lamin B1, which is frequently reduced in senescent cells.

  1. Tchkonia, T. et al. (2013) J Clin Invest 123, 966-72.
  2. Sun, Y. et al. (2018) Trends Mol Med 24, 871-885.
  3. Gorgoulis, V. et al. (2019) Cell 179, 813-827.

Background References

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