Revision 1

#89884Store at -20C

1 Kit

(5 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
MSH2 (D24B5) XP® Rabbit mAb 2017 20 µl 100 kDa Rabbit IgG
MLH1 (D38G9) Rabbit mAb 4256 20 µl 84 kDa Rabbit IgG
MSH6 (D60G2) XP® Rabbit mAb 5424 20 µl 160 kDa Rabbit IgG
PMS2 (E9U4P) Rabbit mAb 27884 20 µl 110 kDa Rabbit IgG
EXO1 (E6P2B) Rabbit mAb 63862 20 µl 120 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Mismatch DNA Repair (MMR) Antibody Sampler Kit provides an economical means of detecting proteins involved in MMR. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


DNA mismatch repair (MMR), a conserved process for detecting and correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity (1). In prokaryotes, a MutS homodimer recruits a MutL homodimer to sites of DNA mismatches. In eukaryotes, six MutS homologues (MSH1-6) and four MutL homologues (MLH1, PMS2, PMS1, and MLH3) have been identified. Heterodimers composed of two MutL homologues detect distinct DNA mismatch lesions, and heterodimers composed of two MutS homologues perform the repair (2). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (1).

Microsatellite instability (MSI) is a predisposition to genetic mutation resulting from MMR deficiency (dMMR). High MSI (MSI-H) arising from dMMR results in Lynch syndrome, also known as hereditary non-polyposis colorectal cancer (HNPCC). Lynch syndrome is associated with colon cancer, as well as other human cancers (3). MSI and dMMR are strongly associated with tumor responsiveness to immune checkpoint blockade (4,5). MSI status can be determined through PCR amplification of microsatellite markers and/or immunohistochemical detection of MMR proteins MLH1, PMS2, MSH2, and MSH6. The absence of expression of any of these MMR proteins indicates dMMR (3).

  1. Modrich, P. (2006) J Biol Chem 281, 30305-9.
  2. Kolodner, R.D. and Marsischky, G.T. (1999) Curr Opin Genet Dev 9, 89-96.
  3. Pećina-Šlaus, N. et al. (2020) Front Mol Biosci 7, 122.
  4. Kok, M. et al. (2019) ESMO Open 4, e000511.
  5. Yi, M. et al. (2018) Mol Cancer 17, 129.

Background References

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