Revision 1

#90298Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Iba1/AIF-1 (E4O4W) XP® Rabbit mAb 17198 20 µl 17 kDa Rabbit IgG
TMEM119 (E3E1O) Rabbit mAb 90840 20 µl Rabbit IgG
ASC/TMS1 (D2W8U) Rabbit mAb 67824 20 µl 22 kDa Rabbit IgG
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl 80 kDa Rabbit IgG
CD11b/ITGAM (M1/70) Rat mAb 46512 20 µl Rat IgG2b kappa
CD45 (30-F11) Rat mAb 55307 20 µl Rat IgG2b kappa
F4/80 (D4C8V) XP® Rabbit mAb 30325 20 µl 65-250 kDa Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129 20 µl Rabbit IgG
CD68 (E3O7V) Rabbit mAb 97778 20 µl 70-80, 130-140, 200 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Mouse Microglia Marker IF Antibody Sampler Kit provides an economical means of detecting proteins identified as microglia markers by immunofluorescence and/or western blot. This kit includes enough primary antibodies to perform at least twenty IF-F tests or two western blot experiments per primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Microglia are the resident macrophages of the central nervous system, responsible for immune response and maintenance of CNS homeostasis (1). Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (2,3). Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (4). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (5). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM) (6). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8-10). Expression of F4/80 has been observed in microglia and subset populations of dendritic cells (11). The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein expressed in all nucleated hematopoietic cells (12). Studies suggest CD45 plays a role in regulation of microglial activation (13,14). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (15,16). It localizes to the lysosomal membrane and is upregulated during microglial activation (17,18). Ki-67 is a nuclear nonhistone protein (19) universally expressed among proliferating cells and absent in quiescent cells (20). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (21). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (22) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (23).

  1. Ginhoux, F. and Prinz, M. (2015) Cold Spring Harb Perspect Biol 7, a020537.
  2. Deininger, M.H. et al. (2002) FEBS Lett 514, 115-21.
  3. Ito, D. et al. (1998) Brain Res Mol Brain Res 57, 1-9.
  4. Bennett, M.L. et al. (2016) Proc Natl Acad Sci U S A 113, E1738-46.
  5. Satoh, J. et al. (2016) Neuropathology 36, 39-49.
  6. Deczkowska, A. et al. (2018) Cell 173, 1073-81.
  7. Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
  8. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  9. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  10. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  11. Greter, M. et al. (2015) Front Immunol 6, 249.
  12. Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
  13. Tan, J. et al. (2000) J Biol Chem 275, 37224-31.
  14. Cosenza-Nashat, M.A. et al. (2006) Brain Pathol 16, 256-65.
  15. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  16. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  17. Wong, A.M. et al. (2005) Neurosci Lett 390, 76-80.
  18. Hendrickx, D.A.E. et al. (2017) J Neuroimmunol 309, 12-22.
  19. Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
  20. Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
  21. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  22. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  23. Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.