Revision 1

#90298Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Iba1/AIF-1 (E4O4W) XP® Rabbit mAb 17198 20 µl 17 kDa Rabbit IgG
TMEM119 (E3E1O) Rabbit mAb 90840 20 µl Rabbit IgG
ASC/TMS1 (D2W8U) Rabbit mAb 67824 20 µl 22 kDa Rabbit IgG
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl 80 kDa Rabbit IgG
CD11b/ITGAM (M1/70) Rat mAb 46512 20 µl Rat IgG2b kappa
CD45 (30-F11) Rat mAb 55307 20 µl Rat IgG2b kappa
F4/80 (D4C8V) XP® Rabbit mAb 30325 20 µl 65-250 kDa Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129 20 µl Rabbit IgG
CD68 (E3O7V) Rabbit mAb 97778 20 µl 70-80, 130-140, 200 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Mouse Microglia Marker IF Antibody Sampler Kit provides an economical means of detecting proteins identified as microglia markers by immunofluorescence and/or western blot. This kit includes enough primary antibodies to perform at least twenty IF-F tests or two western blot experiments per primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


Microglia are the resident macrophages of the central nervous system, responsible for immune response and maintenance of CNS homeostasis (1). Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (2,3). Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (4). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (5). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM) (6). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8-10). Expression of F4/80 has been observed in microglia and subset populations of dendritic cells (11). The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein expressed in all nucleated hematopoietic cells (12). Studies suggest CD45 plays a role in regulation of microglial activation (13,14). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (15,16). It localizes to the lysosomal membrane and is upregulated during microglial activation (17,18). Ki-67 is a nuclear nonhistone protein (19) universally expressed among proliferating cells and absent in quiescent cells (20). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (21). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (22) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (23).

  1. Ginhoux, F. and Prinz, M. (2015) Cold Spring Harb Perspect Biol 7, a020537.
  2. Deininger, M.H. et al. (2002) FEBS Lett 514, 115-21.
  3. Ito, D. et al. (1998) Brain Res Mol Brain Res 57, 1-9.
  4. Bennett, M.L. et al. (2016) Proc Natl Acad Sci U S A 113, E1738-46.
  5. Satoh, J. et al. (2016) Neuropathology 36, 39-49.
  6. Deczkowska, A. et al. (2018) Cell 173, 1073-81.
  7. Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
  8. Hirsch, S. et al. (1981) J Exp Med 154, 713-25.
  9. Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
  10. McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
  11. Greter, M. et al. (2015) Front Immunol 6, 249.
  12. Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
  13. Tan, J. et al. (2000) J Biol Chem 275, 37224-31.
  14. Cosenza-Nashat, M.A. et al. (2006) Brain Pathol 16, 256-65.
  15. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  16. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  17. Wong, A.M. et al. (2005) Neurosci Lett 390, 76-80.
  18. Hendrickx, D.A.E. et al. (2017) J Neuroimmunol 309, 12-22.
  19. Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
  20. Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
  21. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  22. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  23. Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.

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