| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| TCF1/TCF7 (C63D9) Rabbit mAb | 2203 | 20 µl | 48, 50 kDa | Rabbit IgG |
| TCF3/TCF7L1 (D15G11) Rabbit mAb | 2883 | 20 µl | 78 kDa | Rabbit |
| TCF4/TCF7L2 (C48H11) Rabbit mAb | 2569 | 20 µl | 58, 79 kDa | Rabbit IgG |
| LEF1 (C12A5) Rabbit mAb | 2230 | 20 µl | 25-58 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The TCF/LEF Family Antibody Sampler Kit provides an economical means of detecting total protein from the TCF/LEF1 family members. The kit contains enough primary and secondary antibodies to perform two Western blots with each antibody.
Storage
Background
LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1, also known as TCF7), TCF3 (also known as TCF7L1) and TCF4 (also known as TCF7L2) (1). LEF1 and TCF1/TCF7 were originally identified as important factors regulating early lymphoid development (2) that act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).
Background References
Trademarks and Patents
Limited Uses
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