|Bax (D2E11) Rabbit mAb
|Bak (D4E4) Rabbit mAb
|Bok (D7V2N) Rabbit mAb
|Bim (C34C5) Rabbit mAb
|12, 15, 23 kDa
|Bad (D24A9) Rabbit mAb
|15, 22 kDa
|Puma (D30C10) Rabbit mAb
|Noxa (D8L7U) Rabbit mAb
|Anti-rabbit IgG, HRP-linked Antibody
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
The Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit II provides an economical means to examine several members of the Bcl-2 family. The kit contains enough primary antibody to perform two western blot experiments.
The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok; and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.
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