| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb | 7649 | 20 µl | 84, 91 kDa | Rabbit IgG |
| Phospho-Stat2 (Tyr690) Antibody | 4441 | 20 µl | 113 kDa | Rabbit |
| Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb | 9145 | 20 µl | 79, 86 kDa | Rabbit IgG |
| Phospho-Stat3 (Ser727) Antibody | 9134 | 20 µl | 86 kDa | Rabbit |
| Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb | 4322 | 20 µl | 90 kDa | Rabbit IgG |
| Phospho-Stat6 (Tyr641) Antibody | 9361 | 20 µl | 110 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Phospho-Stat Pathway Sampler Kit provides an economical means to evaluate the activation status of Stat molecules, including the phosphorylation of Stat1 at Tyr701, Stat2 at Tyr690, Stat3 at Tyr705/Ser727, Stat5 at Tyr694 and Stat6 at Tyr641. The kit includes enough primary and secondary antibody to perform two Western blot experiments.
Storage
Background
Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).
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- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
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- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
- Bromberg, J. F. et al. (1999) Cell 98, 295-303.
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- Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
- Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
- Frank, D.A. (1999) Mol. Med. 5, 432-456.
Background References
Trademarks and Patents
Limited Uses
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