| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb | 6943 | 20 µl | 60 kDa | Rabbit IgG |
| Non-phospho-Src (Tyr527) Antibody | 2107 | 20 µl | 60 kDa | Rabbit |
| Non-phospho-Src (Tyr416) (7G9) Mouse mAb | 2102 | 20 µl | 60 kDa | Mouse IgG2b |
| Phospho-Src (Tyr527) Antibody | 2105 | 20 µl | 60 kDa | Rabbit |
| Src (32G6) Rabbit mAb | 2123 | 20 µl | 60 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Src Antibody Sampler kit provides an economical means of evaluating total Src protein levels and its phosphorylation status. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.
Storage
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (4).
Lck is essential for T-lymphocyte activation and differentiation (5,6). The activity of Lck is regulated by protein kinases and phosphatases. Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity (7).
- Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
- Yamanashi, Y. et al. (1989) Proc Natl Acad Sci U S A 86, 6538-42.
- Yamanashi, Y. et al. (1991) Science 251, 192-4.
- Molina, T.J. et al. (1992) Nature 357, 161-4.
- Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
- Chow, L.M. et al. (1993) Nature 365, 156-60.
Background References
Trademarks and Patents
Limited Uses
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